Escherichia coli (XL1-BLUE) for continuous fermentation of bioH2 and its separation by polyimide membrane. Int J Hydrog Energy 2012;37:5623-30.Bakonyi, P.; Nemestothy, N.; Ramirez, J.; Ruiz-Filippi, G.; Belafi-Bako, K. Escherichia coli (XL1-BLUE) for continuous fermentation of bio...
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coli XL1-Blue (Stratagene, USA) was used for the antibacterial activity tests. The bacteria were cultured with Luria-Bertani broth (Affymetrix USB) at 37 °C, and washed and suspended in 0.9% NaCl solution before use30. Bacteria and nanoparticles were mixed in a ®suspension inside a ...
coli XL1 Blue were transformed with plasmids pBHR68-PhaP-IL2 and pBHR68-PhaP-MOG, respectively. Transformants were grown at 37°C and induced by adding isopropyl-β-D-thiogalactopyranoside (IPTG) to a final concentration of 1 mM. After growth for 48 h, cells were harvested by ...
σ 70 andσ 70-(1–565)—The wild-type σ70 RNAP subunit was purified from BL21 (DE3) cells harboring the pT7σ plasmid as described (20). To purify C-terminally truncated σ70-(1–565), E. coli XL-1Blue cells were transformed with the pCYB2_σ1–565 plasmid. Transformants were...
TCA cycle by GABA aminotransferase encoded bygabT. When GadA or GadB was overexpressed alone or together with GadC, GadB always showed higher activity. When GadB and GadC were co-overexpressed inE. coliXL1-Blue ΔgabT, 5.46 g/L GABA was obtained from 10 g/L MSG (Le Vo et al.2012...
An efficient bacterial transformation system was described for the preparation of competent cells, plasmid preparation, and for the storage in bacterial stocks. Three different strains of Escherichia coli that were tested were DH5α, TG1 and XL1 blue, and the most efficient strain being XL1 blue....
the two fragments were assembled and transformed by electroporation intoE. coliXL1-Blue. Transformed cells were cultivated on LB agar plates overnight at 37 °C. The library size was estimated to be 105. Plasmids were then extracted and transformed by electroporation intoE. coliMG1655 for screeni...
Purification of pC6-2-expressed GST-free proteins 18| Transform the XL1 blue strain of E. coli competent cells with the pC6-2-cloned construct using the heat-shock method and spread on an LB agar plate supplemented with 100 mg ml–1 ampicillin. m CRITICAL STEP The colonies on the plate ...
coli varied depending upon the source of the PHA synthase and the mutants used. PLA homopolymer could also be produced at ca. 7 wt.% by employing the several PhaC1 variants containing E130D/S325T/S477G/Q481K quadruple mutations in wild-type E. coli XL1-Blue. This is a preview of ...