This article is available as a PDF only.doi:10.1097/00019048-199503000-00012Cieslak, Paul R.Swerdlow, David L.Infectious Diseases in Clinical PracticeCieslak PR, Swerdlow DL. Escherichia coli O157:H7: What the clinicians needs to know. Infectious Diseases in Clinical Prectice; vol 4: 1...
AM Murthy1, AK Fritzsche1, M-D Phan2,3, M Totsika2,4, AAB Robertson1,2, KB Nichols2,3, MA Cooper1,2, KJ Stacey2,3, GC Ulett5, K Schroder1,2, MA Schembri2,3 and MJ Sweet1,2 Uropathogenic Escherichia coli (UPEC) is the main etiological agent of urinary tract infections (UTI...
This prompted the question: What is the role of RpoS at fast population growth rates? To answer this question we analysed periods when cells were growing slower than the optimal threshold for survival (Fig. 4g). The role of RpoS is to prolong the duration of these slow growth events. We ...
B. What Is the Outer Membrane? Before describing the outer-membrane lipoprotein of E. coli, I would like to describe the structure of the E. coli envelope. E. coli, as well as other gram-negative bacteria, is surrounded by an envelope consisting of two distinct membranes as observed by ...
The reaction is dependent upon the presence of the FHL complex in the cells, with a mutant strain (RT2) devoid of the genes encoding the enzyme being unable to generate formate (Figure S3D). Intact E. coli cells are, therefore, under the correct conditions, capable of a highly efficient ...
Shikimic acid (SA) is utilized in the synthesis of oseltamivir-phosphate, an anti-influenza drug. In this work, metabolic engineering approaches were employed to produce SA in Escherichia coli strains derived from an evolved strain (PB12) lacking the phosphoenolpyruvate:carbohydrate phosphotransferase sy...
However, it is not produced by bacterial strains commonly used for industrial biocatalysis or recombinant protein production, such as Escherichia coli, limiting our ability to exploit it as an enzymatic cofactor and produce it in high yield. Here we have utilized a genome-scale metabolic model of...
This review will focus on what is currently known about the third step of the general protein secretion process (SPase I catalyzed cleavage of secretory preproteins) in the model organism Escherichia coli. 2. The substrate–membrane bound secretory preproteins The substrates for SPase I are secreto...
coli strain, harboring the wild-type S. coelicolor PCC, which produced the highest 6-dEB titer, is 1.2%, which is in good agreement with what has been previously reported in literature.29 However, using flux balance analysis, the maximum theoretical molar yield of 6-dEB was calculated to ...
A tantalizing question in evolutionary biology is whether evolution can be predicted from past experiences. To address this question, we created a coherent compendium of more than 15,000 mutation events for the bacterium Escherichia coli under 178 distinct environmental settings. Compendium analysis provi...