coli strain, harboring the wild-type S. coelicolor PCC, which produced the highest 6-dEB titer, is 1.2%, which is in good agreement with what has been previously reported in literature.29 However, using flux balance analysis, the maximum theoretical molar yield of 6-dEB was calculated to ...
AM Murthy1, AK Fritzsche1, M-D Phan2,3, M Totsika2,4, AAB Robertson1,2, KB Nichols2,3, MA Cooper1,2, KJ Stacey2,3, GC Ulett5, K Schroder1,2, MA Schembri2,3 and MJ Sweet1,2 Uropathogenic Escherichia coli (UPEC) is the main etiological agent of urinary tract infections (UTI...
This article is available as a PDF only.doi:10.1097/00019048-199503000-00012Cieslak, Paul R.Swerdlow, David L.Infectious Diseases in Clinical PracticeCieslak PR, Swerdlow DL. Escherichia coli O157:H7: What the clinicians needs to know. Infectious Diseases in Clinical Prectice; vol 4: ...
Herein we report what are to our knowledge the first direct biocatalytic routes to BDO from renewable carbohydrate feedstocks, leading to a strain of Escherichia coli capable of producing 18 g l?1 of this highly reduced, non-natural chemical. A pathway-identification algorithm elucidated multiple ...
coli O157:H7 shedding in North Dakota steers in fall and early spring was comparable to what has been reported in other parts of the United States with relatively warmer weather. Further research into E. coli O157:H7 shedding patterns during extreme weather such as North Dakota winters is ...
coli cells is the prototype of class III ribonucleotide reductases (1). It consists of an α2β2complex, with β2 carrying an essential iron-sulfur center (7). Since β2 after reconstitution binds only 2Fe2+ and 2S2− per polypeptide chain and in the reduced form exhibits an EPR ...
This is based on the fact that control and mutant strains have all exhibited almost the same growth rate with almost the same final OD600). Discussion Decades of work have been dedicated to the biochemical optimization and metabolic engineering of E. coli aiming to increase fatty acids yield ...
This prompted the question: What is the role of RpoS at fast population growth rates? To answer this question we analysed periods when cells were growing slower than the optimal threshold for survival (Fig. 4g). The role of RpoS is to prolong the duration of these slow growth events. We ...
coli bacterial growth, which is partially reduced by the b3023 mutation. Therefore, AI-2 quorum-sensing control of biofilm formation is mediated through regulator MqsR that induces expression of the transcription factor YncC. YncC inhibits the expression of periplasmic YbiM, which prevents ...
Shikimic acid (SA) is utilized in the synthesis of oseltamivir-phosphate, an anti-influenza drug. In this work, metabolic engineering approaches were employed to produce SA in Escherichia coli strains derived from an evolved strain (PB12) lacking the phosphoenolpyruvate:carbohydrate phosphotransferase sy...