W3110价格:2800元 代购价格:询价 联系方式:I47-825O-882O 相关技术服务:微生物基因敲除和回补立刻购买 买家导航 W3110标准菌株基本信息 出品公司: ATCC 菌种名称: W3110 菌种又名: W3110大肠杆菌 菌株类型: 大肠杆菌,Escherichia coli 存储人: Joshua Lederberg, Dept. of Genetics, School of Medicine, ...
A four-carbon dicarboxylic acid L-malate has recently attracted attention due to its potential applications in the fields of medicine and agriculture. In this study, Escherichia coli W3110 was engineered and optimized for L-malate production via one-step L-malate synthesis pathway. First, deletion...
再将纯化后的打靶片段通过电击转化导入到E.coliW3110中,经菌落PCR验证,如图4A所示,片段1～3大小与阳性对照一致,证明Kan抗性成功替代了E.coliW3110菌株染色体上醛脱氢酶的基因位置;之后通过导入pCP20质粒消除Kan
X Dong,X Chen,Y Qian,Y Wang,L Wang,W Qiao,L Liu 摘要: A four-carbon dicarboxylic acid L-malate has recently attracted attention due to its potential applications in the fields of medicine and agriculture. In this study, Escherichia coli W3110 was engineered and optimized for L-malate ...
coli8,9. Since 1,3-DAP , a three carbon diamine, might also be toxic to E. coli host, the tolerance of E. coli to 1,3-DAP was first assessed prior to strain devel- opment. Tolerance was tested by monitoring the growth profile of wild-type E. coli W3110 strain in R/2 medium ...
W3110 is an E. coli wild type strain, employed in this study as a parental host [19]. VH33 is a PTS- glucose+ strain derived from W3110, which recovered the ability to grow on glucose as a result of replacing the native promoter region of galP in the chromosome by the strong trc ...
Escherichia coli TC44, a derivative of W3110, was engineered for the production of pyruvate from glucose by combining mutations to minimize ATP yield, cell growth, and CO2 production (DeltaatpFH DeltaadhE DeltasucA) with mutations that eliminate acetate production [poxB::FRT (FLP recognition targ...
(2006). Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol U95–U99. Hengge R. (2011). The General Stress Response in Gram- Negative Bacteria. ASM Press: Washington DC, USA, pp 251–289. Houndt T, Ochman H. (2000). Long-term ...
coli W3110 strains were named W-Ah and W-Vv, respectively. These strains were cultured in M9 minimal medium supplemented with glucose or glycerol, and we added p-coumaric acid as a precursor for resveratrol, given that these strains do not express a TAL enzyme. In this experiment, we ...
coli W3110 that is similar to that of WbaP2,6, as explained in the Introduction. Numbers next to each predicted transmembrane helix (TMH) indicate the position of amino acid residues at the predicted boundaries between soluble and membrane embedded regions. TMHs are indicated by roman numerals....