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Escherichia coliMG1655CRISPR‐Cas9Defense systemMetabolic engineeringEscherichia coli strain K-12 MG1655 has been proposed as an appropriate host strain for industrial production. However, the direct application of this strain suffers from the transformation inefficiency and plasmid instability. Herein, we ...
Strain MG1655+ hisG r hisL′-ΔΔ, purR, which produces histidine with a weight yield of approximately 12% from glucose, was constructed through directed chromosomal modifications of the laboratory Escherichia coli strain MG1655+, which has a known genome sequence. A feedback-resistant ATP-...
coli strains to colonize the mouse large intestine has been correlated with their ability to grow in cecal and colonic mucus. In the present study, an E. coli MG1655 strain was mutagenized with a mini-Tn5 Km (kanamycin) transposon, and mutants were tested for the ability to grow on agar...
Escherichia coli MG1656 MG1655 (K-12F– λ– ilvG– rfb-50 rph-1) ΔlacMluI Escherichia coli MG1656 λatt::gfp MG1656 strain containing the gfpmut3 gene at the λatt site Escherichia coli MG1656 Δcpxl::km-frt cpxl gene replaced by the km-frt cassette in strain MG1656 Espéli et...
MG1655大肠杆菌,Escherichia coli MG1655,SHBCC D24975,MG1655 公司简介 上海瑞楚生物科技有限公司(www.ruichubio.com)是一家以科技为驱动力,以市场为导向的科技型企业,公司产品依托江苏工厂(瑞楚生物科技(江苏)有限公司)负责生产。公司目前拥有自我品牌"瑞楚生物REBIO",其业务核心是利用AI驱动自动化设备对微生物进...
In previous studies, we have shown the existence of metabolic remodeling in glucose-grown EscherichiacoliMG1655 cells expressing the esterase Orf306 from the opd island of Sphingobiumfuliginis. We now show that Orf306-dependent metabolic remodeling is due to regulation of a novel small RNA (sRNA)...
coli strains to colonize the mouse large intestine has been correlated with their ability to grow in cecal and colonic mucus. In the present study, an E. coli MG1655 strain was mutagenized with a mini-Tn5 Km (kanamycin) transposon, and mutants were tested for the ability to grow on agar...
coli genome has about the same ratio of coding regions (e.g., the MG1655 strain has 86% of its DNA in coding regions), the position of the fixated mutations is not biased towards either intergenic or genic regions. Transcription-related genes such as rpoB are among the top genes detected...
Complete NotI, SfiI, XbaI and BlnI cleavage maps of Escherichia coli K-12 strain MG1655 were constructed. Techniques used included: CHEF pulsed field gel electrophoresis; transposon mutagenesis; fragment hybridization to the ordered $\\l... Perkins,J Dean 被引量: 0发表: 1993年 New tools for...