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on LB agar plates. The colonies on the plates were counted after an overnight incubation at 37 °C to determine the CFU. Survival fraction was computed as cells/mL from the stress condition divided by the cells/mL from the water condition. Averages were taken over all plates that were ...
g, Spot assay for the indicated strains overexpressing ybaB, recR, or a control empty plasmid after exposure to UV with a total energy of 85 mJ cm−2 or control non-exposed plate. Cells were diluted to OD578 = 0.1 and then serially diluted in tenfold steps, and spotted on LB agar ...
coli BL21DE3/pBJN406 were grown analogically to the CHO cells adherence experiments, on agar plates containing 3.5 mM pilicide. The fully-fimbriated bacteria grown in the absence of pilicide (positive control) resulted in an HA-titer of 128. The non-fimbriated bacteria E. coli BL21DE3/...
Intracellular precursor supply is a critical factor for amino acid productivity. In the present study, ppsA and tktA genes were overexpressed in genetically engineered Escherichia coli to enhance the...
The effectiveness of solar disinfection (SODIS), a low-cost household water treatment method for developing countries, was investigated with flow cytometry and viability stains for the enteric bacterium Escherichia coli. A better understanding of the process of injury or death of E. coli during SODIS...
Diffusely adherent Escherichia coli (DAEC) have been considered a diarrheagenic category of E. coli for which several potential virulence factors have been described in the last few years. Despite this, epidemiological studies involving DAEC have shown i
This paper describes a novel single-tube agar-based technique for motility enhancement and immunoimmobilization of Escherichia coli O157:H7. Motility indole ornithine medium and agar (0.4%, wt/vol) media containing either nutrient broth, tryptone broth, or tryptic soy broth (TSBA) were evaluated ...
Each strain was streaked out on an agar plate containing minimal medium supplemented with 500 mM methanol and incubated at 37 °C until colonies were clearly visible. A cross-section of the colonies was used to inoculate a pre-culture in a 30 ml volume of minimal medium supplemented ...
The generation time for Escherichia coli K-12 in deuterated medium is substantially increased compared to cells grown in hydrogenated (protiated) medium. By using a mutagenesis plasmid based approach we have isolated an E. coli strain derived from E. coli K-12 substrain MG1655 that show ...