An Escherichia coli isolate was recovered from a 92-year-old female patient with urinary tract infection. Gram-stained preparation of the urine sediment manifested some gram-negative rod-shaped cells, and the urine specimen culture yielded nonhemolytic colonies on sheep blood ag...
contrast microscopy and indirectly by hemagglutination; adherence to peripheral blood leukocytes, using radiolabeled bacteria and subsequent determination of leukocyte-associated radioactivity; and adherence to renal glomeruli, by microscopy of fluoresceinated bacteria and of Gram-stained nonfluoresceinated ...
coli isolates was done based on colony morphology, Gram stain and growth on MacConkey agar and eosin methylene blue (EMB) agar (Sigma, Beijing, China) [5]. All suspected isolates were later confirmed as E. coli by sequencing of 16S rDNA [14]. Confirmed E. coli isolates were stored in ...
Here, we measure the growth of Escherichia coli flagella in real time and find that, although the growth rate displays large variations at similar lengths, it decays on average as flagella lengthen. By tracking single flagella, we show that the large variations in growth rate occur as a ...
We next asked whether theE.coli-containing phagosomes bore the late endosomal/lysosomal marker Lysosomal Associated Membrane Protein 1 (LAMP1). For this purpose, activated macrophages were infected with a MOI of either 20 of Anc or 120 of M6 bacteria. MΦs were fixed and stained at one or ...
Probiotic Escherichia coli Nissle 1917 (EcN) has been widely studied for the treatment of intestinal inflammatory diseases and infectious diarrhea, but the mechanisms by which they communicate with the host are not well-known. Outer membrane vesicles (OMVs) are produced by Gram-negative bacteria and...
Diffusely adherent Escherichia coli (DAEC) have been considered a diarrheagenic category of E. coli for which several potential virulence factors have been described in the last few years. Despite this, epidemiological studies involving DAEC have shown i
Diffusely adherent Escherichia coli (DAEC) have been considered a diarrheagenic category of E. coli for which several potential virulence factors have been described in the last few years. Despite this, epidemiological studies involving DAEC have shown inconsistent results. In this work, two different...
coli biofilm formation. Biofilms were grown in non-treated multi-well plates for 48 h and subsequently stained with crystal violet. Absorbance at 595 nm was measured and normalized to APE- values (n = 3 repeats; error bars represent SD; ****p < 0.0001 by unpaired, two-...
The type III secretion system (T3SS) is a multi-protein complex that plays a central role in the virulence of many Gram-negative bacterial pathogens. In enteropathogenic Escherichia coli, a prevalent cause of diarrheal diseases, the needle complex base of the T3SS is formed by multi-rings: two...