OMVs encapsulate material of cytoplasmic and periplasmic origin, like DNA, RNA or proteins. However, the exact composition of the OMVs varies between different species and strains of bacteria. It was also shown that OMVs fromE. coliincorporate approximately 1% of their outer membrane material includin...
Support for a helical hairpin arrangement of TatA was also provided by Chan et al. (28), who showed that in whole cells cysteine residues in the APH or C terminus of TatA were not labeled by a membrane-impermeable thiol reagent. They further showed that in the presence of an uncoupler ...
coli strain E2348/69. The purified protein was structurally near-identical to the wild-type protein. In bovine T lymphocytes treated with wild-type LifA, a putative cleavage product of approximately 140 kDa was detected. Appearance of the putative cleavage product was inhibited in a concentration-...
6.2. Arrangement of Glycan Chains In collaboration with the group of the late Uli Schwarz in Tübingen, we isolated purified sacculi from E. coli and incubated them with enzymes that disrupt peptidoglycan. Thereafter, they were prepared for electron microscopy. Sacculi became fully disrupted after ...