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coli are gray, smooth, and often beta﹉emolytic on blood agar. Hemolytic colonies isolated from urine specimens usually suggest more virulent strains. MacConkey agar and either Hektoen enteric or xylose lysine deoxycholate agar are recommended for optimal isolation of Shigella. The genus Salmonella ...
E. coli isolation. Fecal samples were streaked onto MacConkey agar (Difco, Sparks, Maryland) and incubated at 37°C for 18 h without antimicrobials, after which five lactose-positive colonies were selected (27). The colonies were transferred to Chromocult coliform agar (Merck KGaA, Darmstadt,...
The dipslide consisted of cysteine-, lactose, and electrolyte-deficient (CLED) agar on one side and MacConkey agar on the other side. Inoculation was done by the study nurse: dipslides were fully submerged in the urine sample, alternatively, a sterile pipette was used to inoculate both agar...
coli populations were enumerated on MacConkey (MC) and MC-supplemented media, and eight virulence-associated genes (VAGs) (iroN, iutA, iss, ompT, hlyF, vat, frzorf4 , and fyuA) were sought by PCR on isolates obtained on MC agar. The results showed significant between-group differences ...
Thirty seven strains showed more than 105cfu/ml urine sample on MacConkey agar medium and EMB agar medium. E. coli strains showed specific morphological, cultural and biochemical characteristic of E. coli. The increase in incidence of bacteriuria in female subjects was observed in all three age ...
coli using the disk diffusion method have been described previously [3]. Briefly, suspected dark pink E. coli colonies on MacConkey agar were streaked on Eosin Methylene Blue agar and incubated at 37 °C for 24 h. Isolates were confirmed as E. coli using Microbact GNB 24E (Oxoid, UK)....
H Nicoloff,V Perreten,SB Levy 摘要: Thirteen spontaneous multiple-antibiotic-resistant (Mar) mutants of Escherichia coli AG100 were isolated on Luria-Bertani (LB) agar in the presence of tetracycline (4 microg/ml). The phenotype was linked to insertion sequence (IS) insertions in marR or ...
The test is best used in conjunction with Sorbitol MacConkey Agar (Oxoid CM0813). E. coli O157:H7 strains do not ferment Sorbitol and therefore give colourless colonies on this medium. The majority of E coli isolates do ferment Sorbitol and give characteristic pink colonies. Sorbitol MacConkey ...
The samples were cultured on MacConkey agar plates and colonies were identified by standard biochemical methods. Antibiotic sensitivity testing of the isolates against 13 antibiotics was performed according to the CLSI guideline using the disk diffusion method. Among stool and clinical samples, more ...