DH5α是大肠杆菌(Escherichia coli)的一种感受态细胞系,通常被用于分子生物学实验中。这些细胞被设计成对外源DNA接受性较高,因此在分子克隆、DNA转化和质粒扩增等实验中被广泛使用。DH5α感受态细胞对于吸收外源DNA特别有效,这使得它们在基因工程和分子生物学研究中成为常见的实验室工具。中文名称:DH5a感受态细胞 英文...
Background Plasmid propagation in recombination strains such as Escherichia coli DH5α is regarded as a beneficial instrument for stable amplification of the DNA materials. Here, we show trans-conjugation of pGEM-T cloning vector (modified Promega PCR product cloning vector with tra genes, ...
英文名 Escherichia coli DH5α(pEX18TC) 相关类别 质粒 储存 冷藏(2-8℃) 编号 包装 库存 目录价(¥) 您的价格(¥) 数量 A339308-0001 1 VIA 咨询 1420 1420产品描述 概述 本产品/服务仅供科研使用 应用 1.准备1支含有5~10mL液体培养基的试管和2个平板;2.安全柜中打开,用酒精灯灼烧顶部,后迅速滴...
上海玉博生物科技有限公司 主要致力于“Anti-E.coli DH-5α (Escherichia coli DH-5α ) 抗DH5α大肠杆菌菌体蛋白抗体 yb-2033R”的生产销售。多年的“Anti-E.coli DH-5α (Escherichia coli DH-5α ) 抗DH5α大肠杆菌菌体蛋白抗体 yb-2033R”生产与销售的经验,与各行业
DH5α(含PSH65质粒)大肠杆菌英文名 Escherichia coli DH5α(PSH65) 相关类别 质粒 储存 冷藏(2-8℃) 编号 包装 库存 目录价(¥) 您的价格(¥) 数量 A339194-0001 1 VIA 咨询 829 829产品描述 概述 PSH65质粒提取本产品/服务仅供科研使用 应用 1.准备无菌水1支,90mm抗性平板2块; 2.安瓿管表面消毒后...
Bacterial trans-conjugation was used as a tool to activate β-galactosidase productivity through alpha complementation between pGEM harboring E. coli LK111 and recipient E. coli DH5α (∆M15lacZ). Active trans-conjugant isolates further improved its productivity through UV mutagenesis, and M-KH-...
Effect of carbon source addition on toluene biodegradation by an Escherichia coli DH5alpha transconjugant harboring the TOL plasmid. Biotechnol Bioeng 2010 ; 107:269 - 77; http://dx.doi.org/ 10.1002/bit.22808 ; PMID: 20506384Ikuma K, Gunsch CK (2010) Effect of carbon source addition on ...
In this communication, biosynthesis of gold nanoparticles assisted by Escherichia coli DH5α and its application on direct electrochemistry of hemoglobin are reported. The gold nanoparticles formed on the bacteria surface are mostly spherical. The direct electrochemistry of hemoglobin can be achieved by ...
coli DH5α were 15 and 42.1 mg mL 1 , respectively. The experimental results revealed that the toxicity of the Co(II) compound was larger than that of Co(sep) 3+ . On the other hand, the scanning electron microscopy (SEM) demonstrated that the two cobalt compounds had the same toxic ...
In this study, a new semi-defined formulated medium was shown to be useful for high cell density cultivation of Escherichia coli DH5α (ATCC 53868). Comprising K 2 HPO 4 , 12.54; KH 2 PO 4 , 2.31; D-Glucose, 4.0; NH 4 Cl, 1.0; Yeast extract, 12.0; NaCl, 5.0; MgSO 4 , 0.24...