且这些作用可被ERK激活性蛋白激酶 (MEK)抑制剂PD98059抑制(均P〈0.01);反PAR-2激动肽VKGILS—NH2对HepG2细胞增殖能力的影响不明显,与对照组相比差 异无统计学意义(均P〉0.05).结论肝癌HepG2细胞表达PAR-2.胰蛋白酶,SLIGKV—NH,可以通过激活PAR-2诱导HepG2细胞的 增殖活性,且该过程部分由ERK/AP.1信号...
且这些作用可被ERK激活性蛋白激酶(MEK)抑制剂PD98059抑制(均P<0.01);反PAR-2激动肽VKGILS-NH_2对HepG2细胞增殖能力的影响不明显,与对照组相比差异无统计学意义(均P>0.05).结论 肝癌HepG2细胞表达PAR-2.胰蛋白酶,SLIGKV-NH_2可以通过激活PAR-2诱导HepG2细胞的增殖活性,且该过程部分由ERK/AP-1信号通路所...
目的 探讨低盐培养对小鼠致密斑(mouse macula densa derived cells,MMDD1)细胞环氧化酶-2(cyclooxygenase,COX-2)表达和p44/42 激酶(ERK), AP-1活性的影响.方法 经脂质体转染含AP-1的报告质粒,采用瞬时表达方法检测AP-1转录...
(2015). "Antroquinonol from antrodia camphorata suppresses breast tumor migration/invasion through inhibiting ERK-AP-1-and AKT-NF-κB-dependent MMP-9 and epithelial- mesenchymal transition expressions." Food and Chemical Toxicology.Lee, Wai-Theng,Lee,etc.Antroquinonol from Antrodia Camphorata suppresses ...
ERK1/2 Antibody Western Blot validation (1/5000 dilution) in HT29 (Cat no:11257-1-AP) FH XB (Verified Customer) (10-29-2020) A reliable ab to detect total ERK1/2 by Western blotting. Applications:Western Blot Primary Antibody Dilution:1:3000 ...
方法: (1)本研究中,从健康青少年包皮组织中提取人皮肤角质形成细胞.采用不同剂量UV/IPL照射人皮肤角质形成细胞, 15mins时WB法检测p-ERK,p-P38和p-JNK蛋白水平,筛选出最佳照射剂量;采用 UV100mJ/cm2和17J/cm2IPL分别照射人皮肤角质形成细胞,照射后不同时间点用WB法检测p-ERK,p-P38和p-JNK蛋白水平,筛选出...
WB analysis of NIH/3T3 using 16443-1-AP Various lysates were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.View All Images (12)ERK...
结论:(1)细胞水平:IPL照射光老化人皮肤角质形成细胞,抑制ERK,活化JNK,继而活化c-jun和c-fos,抑制MMP和上调Cyclin D1表达,前者减少胶原降解,后者促进角质形成细胞增殖,从而达到治疗皮肤光老化的效果,与UV导致的MAPK/AP-1/MMP表达模式不同.(2)IPL照射后豚鼠光老化皮肤表皮厚度增加,c-fos,c-jun活化,MMP-1,MMP-...
Cited in 400 publications. ERK1/2 antibody for WB, IHC, FC (Intra), ELISA and reacts with human, mouse, rat.
Various lysates were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. -/- Cat No.16443-1-AP Freight/Packing:- Country/Region Size/Concentration ...