Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of ...
produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain ...
This phospho-VRK3 antibody specifically recognized wild-type VRK3 when it was phosphorylated by CDK5 at Ser 108 and a phosphomimetic mutant of Ser 108 (GST-VRK3S108E) but did not recognize the S108A mutant (Supplementary Fig. S1a–e). The phosphorylation signal in the wild-type VRK3 was ...
(Cell Signaling Technology, cat # 2138), recombinant monoclonal rabbit anti-HSL (clone D6W5S; Cell Signaling Technology 4107), polyclonal goat anti-MGL (Abcam, cat # ab77398), recombinant monoclonal rabbit anti-FASN (clone C20G5; Cell Signaling Technology, cat #3180), polyclonal rabbit anti...
Monoclonal antibodies to hemagglutinin (HA) and c-Myc were from Babco. Antibody to active ERK was from Promega and antibody to phospho-Akt was from New England Biolabs. Anti-mouse IgG conjugated with Cy3 was from Jackson ImmunoResearch Laboratories. Cytosine arabinoside (araC) and Hoechst 33342 ...
Sections were then subjected to immunohisto- chemical analysis as previously described.40 Briefly, the sections were stained with rabbit monoclonal antibody against cleaved Caspase3 (Asp175; Clone 5A1E; Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal antibody against GFP (ThermoFisher...
Pretreatment of cells for 30 min with anti-αvβ3 but not anti-αvβ5 inhibitory monoclonal antibody markedly inhibited CYR61-induced BMP-2 mRNA and protein expression using qPCR analysis (Fig. 5A), ELISA assay, and Western blot analysis (Fig. 5B). Treatment with cyclic (RGD) peptides, ...
encoded latent membrane protein 1 (LMP1). In the present study, we used NPC cell lines as models and found that LMP1-augmented kappa production corresponds with elevations in ERKs phosphorylation. PD98059 attenuates LMP1-induced ERKs phosphorylation resulting in decreased expression of the kappa light...
Insulin is a promising drug for the treatment of diseases associated with brain damage. However, the mechanism of its neuroprotective action is far from being understood. Our aim was to study the insulin-induced protection of cortical neurons in oxidativ