Scientists have developed two powerful tools for RNA sequencing (RNA-Seq) experiments to minimize bias and variability: unique molecular identifiers (UMIs) and ERCC RNA spike-ins. We’ll cover the basics of each and h...
ERCC的RNA spike-in有什么作用? ERCC的全称是 External RNA Controls Consortium,翻译过来就是外源RNA参照协会,这是个专门为了定制一套spike-in RNA而成立的组织。该组织成立于2003年,主要的工作就是设计了一套非常好用的spike-in RNA,方便当时的microarray,qRT-PCR以及后来的RNA-Seq进行内参定量。 早期的用DNA芯片...
* 尽管 ERCC RNA 内参混合物1和 ExFold 内参混合物1中包含的 ERCC 转录本配方相同,但 ERCC RNA 内参混合物1不能取代 ExFold 内参混合物1进行倍数变化评估。仅可使用生产批号相同的 ExFold 内参混合物1和混合物2。 仅供研究使用。不适用于诊断用途。
The Ion AmpliSeq RNA ERCC Companion Panel consists of 10 primer pairs targeting 10 transcripts from the ERCC RNA Spike-In Mix representing a 13log2 dynamic range. Chosen for general GC balance and amenability for linear amplification (TaqMan qPCR), the selected ERCC targets produce R2 > 0.9 un...
External RNA Controls Consortium (ERCC) ERCC是啥? 外部RNA控制联盟,就是一套RNA-seq,基因表达检测过程中的控制系统,使得结果具有可重复性。 RNA Spike-in Controls for Gene Expression While early gene expression measurements with DNA microarrays were groundbreaking in their ability to reveal biological ...
本发明的该实施方案包括,第一,一种确实可靠的,从FPE样品中提取RNA的方法,第二,通过使用一对寡核苷酸引物,使用逆转录酶聚合酶链式反应测定样品中ERCC1 mRNA含量的方法,其中优选寡核苷酸引物对ERCC1-504F(SEQ ID NO1)和ERCC1-574R(SEQ ID NO2),或基本上与其相同的寡核苷酸。RNA是通过1999年12月20日提交的美国...
本发明的该实施方案进一步包括,通过使用一对寡核苷酸引物,使用逆转录酶聚合酶链式反应测定样品中ERCC1 mRNA含量的方法,其中优选寡核苷酸引物对TS-763F(SEQ ID NO3)和TS-825R(SEQ IDNO4)或基本上与其相同的寡核苷酸。RNA是通过1999年12月20日提交的美国专利申请09/469,338中描述的任意mRNA分离的方法从FPE细胞中提...
RNA-seq data were corroborated by RT-qPCR and immunoblotting experiments. There is much interest in combination approaches that target epigenetic 'readers, writers, and erasers' that are deregulated in cancer and other pathologies. The current work identified promising second-generation inhibitors with ...
S1N). This approach revealed no consumption of the RNA oligo. Thus, XAB2 does not bind directly to but is in complex with UsnRNAs likely due to its interaction with, e.g., pre-mRNA-splicing factors. RNA-Seq analysis in siXab2 HEPA cells and mESCs 48 h post-transfection revealed ...
The use of Akkermansia muciniphila as potential therapeutic intervention is receiving increasing attention. Health benefits attributed to this bacterium include an improvement of metabolic disorders and exerting anti-inflammatory effects. The abundance o