Background: Mitochondria and endoplasmic reticulum (ER) contact sites (MERCS) constitute a functional communication platform for ER and mitochondria, and they play a crucial role in the lipid homeostasis of the liver. However, it remains unclear about the exact effects of MERCs on the neutral ...
我们已经知道MECP与细胞内多个蛋白相互作用,例如蛋白复合物Mitochondrial Contact Site and Cristae Junction(MICOS)和内质网上的钙离子传感器IP3R。然而,我们对于这些相互作用的具体机制和其对细胞功能的影响了解有限。未来的研究可以通过蛋白质相互作用研究的方法,探索MECP与其他蛋白质的关系,从而揭示其对细胞功能的调控...
Here, using super-resolution fluorescence microscopy, we report that Miro proteins form nanometer-sized clusters along the mitochondrial outer membrane in association with the Mitochondrial Contact Site and Cristae Organizing System (MICOS). Using knockout mouse embryonic fibroblasts we show that Miro1...
(MDT) activities that predominantly occur at the ER-mitochondria contact sites (EMCS). We further demonstrate that a mitochondrial inner membrane protein complex MICOS links nucleoids to Miro1, a KIF5B receptor on mitochondria, at the EMCS. We show that such active transportation is a mechanism ...
Mitochondria-ER contact sites (MERCS) are involved in energy homeostasis, redox and Ca2+ signaling, and inflammation. MERCS are heavily studied; however, little is known about their regulation during mitosis. Here, we show that MERCS expand during mitosis in three cell types using various approache...
ER-mitochondria contact sites represent hubs for signaling that control mitochondrial biology related to several aspects of cellular survival, metabolism, cell death sensitivity and metastasis, which all contribute to tumorigenesis. Altered ER-mitochondria contacts can deregulate Ca2+ homeostasis, phospholipid...
These cancer cells can gain resistance against cell death by modifying the ER-mitochondria contact [26, 28]. Thus, high expression of GRP75 in OC contributes to CP-resistance through the exact mechanism that is a mystery. Although MAM integrity is required for insulin signaling and is ...
The sites of near-contact between the mitochondrion and the endoplasmic reticulum (ER) have earned a lot of attention due to their key role in the maintenance of lipid and calcium (Ca2+) homeostasis, in the initiation of autophagy and mitochondrial divis
Mitochondria-ER contact sites (MERCS) are known to underpin many important cellular homoeostatic functions, including mitochondrial quality control, lipid metabolism, calcium homoeostasis, the unfolded protein response and ER stress. These functions are known to be dysregulated in neurodegenerative diseases...
2I, L, where the SPLICS puncta were quantified on 3D rendered confocal stacks, the number of ER-mitochondria contact sites was significantly decreased in HeLa cells overexpressing the E protein, while no changes were measured upon overexpression of the M protein (values ± SEM: 84.99 ±...