i, j Steady-state [Ca2+]ER in the cultured CGNs infected with a ratiometric [Ca2+]ER probe (ER-GCaMP6-150) was measured by fluorescent signal ratio of ER-GCaMP6-150 to DsRed (i). Data summary of [Ca2+]ER between the indicated genotypes (j). k, l CLCC1 expression level and ...
Cells expressing ER-GCaMP6–150 were transferred to the imaging chamber without any additional pretreatment. Cells were imaged at room temperature in a plastic perfusion chamber in HEPES-Ringer under continuous perfusion with the help of a Minipuls 3 peristaltic pump (speed of the perfusion pump:...
proteins and treated with 1 μM doxo for 24 h.gWestern blot analysis of lysates from REEP1/2 double knockout U2OS cells transfected with the vector, REEP1 or REEP1ΔH2 and treated with 1 μM doxo for 24 h.hWestern blot analysis of wild-type and EI24-knockout U2OS cells tr...
研究团队使用基因编码钙指示剂(GCaMP)对狨猴的初级视觉皮层(V1)和更高级视觉区域进行成像,分析两种活动之间的关系。在初级视觉区域,自发活动和刺激诱发活动表现出高度相似的模式,而在高级视觉区域,这两种活动逐渐变得正交(即彼此独立)。这种正交化(orthogonalization)过程显示出大脑分离信号与噪声的层次性机制。这些结果不...
TRPML1-Gcamp6 expressing primary neurons On the day of isolation, neurons were seeded at 1.5∗105 cells on 3 cm poly-L-Lysine glass coverslips (41001130, Circular 1001/30, Hecht-Assistent, Germany), and were co-cultured with a glial feeder layer (see hippocampal neuron culture). Recording...