Weigh 0.5711 g Na2HPO4 (Mr 141.96 g/mol), 0.24 g KH2PO4 (Mr 136.09 g/mol), 8 g NaCl (Mr 58.44 g/mol), and 0.2 g KCl (Mr 74.55 g/mol) and dissolve in ~900 mL distilled water. Adjust to pH 7.4 with 1 M HCl and make up to 1000 mL with distilled water. • Oxidi...
The equivalent unit was introduced to account for the fact that when solutes dissolve in solvent to create a solution, the number of particles dispersed depends on the valence of the solute. For example, when one molecule of KCl dissolves, it leaves two ions, or charged particles − a K+...
second and third coordination shells, which are the structural signatures of neat water under high pressures, are not present in the FWs of NaCl solutions. Rather similar pictures are also seen in the simulated KCl and NaBr solutions, the details of which are presented in Supplementary Discussion...
darkened and re-illuminated plants. Leaves were infiltrated with 66 mM potassium phosphate buffer (pH 7.0) containing 10 mM MgCl2, 100 mM KCl, 0.2 mM DTPA and 2 mg ml−1DTT. Apoplast washing fluid
F1F0 ATPase was isolated and purified following a slightly modified procedure of Ishmukhametov et al. [6]. Briefly, 5 g cells were resuspended in 20 ml lysis buffer (200 mM Tris–Cl pH 7.8, 100 mM KCl, 5 mM MgCl2, 0.1 mM K2-EDTA and 2.5% glycerol) and subsequently passed twice thro...
Controls received either sham ischaemia (n = 6) or cerebral ischaemia (n = 6) followed by an equivalent ICV infusion of vehicle (artificial cerebrospinal fluid: KCl 5 mmol/L, NaCl 137 mmol/L, CaCl2 0.8 mmol/L, 0.1% bovine serum albumin, pH 7.4). The ICV infusion ...