Number of EpCAM-enriched CTCs and potential CTCs/double positive events (CKpos/CD45pos) within the EpCAM-depleted fractions in blood of breast cancer patients.Helen, SchneckBerthold, GierkeFrauke, UppenkampBianca, BehrensD...
Scoring of CTC. The CellSearch cartridges were analyzed twice, first to identify the traditional mark- ers (DAPI+ , CK-PE+ , CK-FITC–, CD45–) and a dual positive CTC definition (DAPI+ , CK-PE+ , CK-FITC+ , CD45–) after which the sample was reanalyzed for only...
strongly supporting our hypothesis that rVAR2 efficiently captures CTCs with an intermediate epithelial and mesenchymal phenotype (Fig.4i). Notably, a minor fraction of the double-negative cells (CK−, CD45−, DAPI+) was found to be vimentin positive, suggesting the presence of mesenchymal CK...
This preference is largely attributed to CELLSEARCH being the first FDA-approved technique for detecting CD45-negative, EpCAM-positive, and cytokeratin 8-, 18-, and/or 19-positive CTCs in peripheral blood [1]. The clinical relevance of EpCAM-positive CTC detection has been substantiated in ...
[122], with interleukin-2 to activate T cells in the vicinity of EpCAM-positive tumor cells (huKS-IL2) [123], and with encapsulated inhibitory RNAs [124,125,126]. In 2015, a phase I clinical trial of patients with EpCAM-positive metastatic cancers aimed at evaluating the maximum ...
b CD45 −CD31− cells were analyzed for EpCAM expression and SSC (side scatter) to sort EpCAM+ cells (left). The sorted cells were reanalyzed (right). c Immunofluorescence analysis of cytospin preparations of sorted P1 cells. Almost all cells were positive for proSP-C expression. Scale...
“false positive” CTCs were detected in 1 subject according to the CTC definition of DAPI+/CK+/CD45−cells (Fig.3(A)). We think that this may have been caused by the contamination of normal vascular endothelial cells during the process of venepuncture, which also reflects the current ...
At the same time, PAK4 exhib- ited higher expression levels in triple-positive (CD24+/ CD44+/EpCAM+) pancreatic CSCs than in triple-nega- tive CSCs [72]. As previously stated, hypoxia was found to increase the N-glycosylation of EpCAM in BC, which induced the nuclear translocation...
CD45 surface marker-positive leucocytes (green colour dot images) (FITC-labelled CD45 antibody, eBioscience, San Diego, CA, USA), calcein AM-positive live cells (orange colour dot images) (Thermo Fisher Scientific, Waltham, MA, USA), and Hoechst-positive nucleated cells (blue colour dot imag...