Number of EpCAM-enriched CTCs and potential CTCs/double positive events (CKpos/CD45pos) within the EpCAM-depleted fractions in blood of breast cancer patients.Helen, SchneckBerthold, GierkeFrauke, UppenkampBianca, BehrensD...
Scoring of CTC. The CellSearch cartridges were analyzed twice, first to identify the traditional mark- ers (DAPI+ , CK-PE+ , CK-FITC–, CD45–) and a dual positive CTC definition (DAPI+ , CK-PE+ , CK-FITC+ , CD45–) after which the sample was reanalyzed for only...
strongly supporting our hypothesis that rVAR2 efficiently captures CTCs with an intermediate epithelial and mesenchymal phenotype (Fig.4i). Notably, a minor fraction of the double-negative cells (CK−, CD45−, DAPI+) was found to be vimentin positive, suggesting the presence of mesenchymal CK...
The total count of CTCs was determined to be 239 cells across 20 samples.EPCAM-negative CTCs were identified as cells with no expression of thePTPRC(CD45) andEPCAMgenes, along with an expression level of epithelial genes (KRT7,KRT8,KRT18) exceeding 0. Conversely,EPCAM-positive CTCs were id...
Furthermore, EpCAM-specific antibodies were conjugated with bouganin (citatuzumab bogatox) [120], Pseudomonas exotoxin A (oportuzumab monatox) [121] and alpha-amanitin [122], with interleukin-2 to activate T cells in the vicinity of EpCAM-positive tumor cells (huKS-IL2) [123], and ...
b CD45 −CD31− cells were analyzed for EpCAM expression and SSC (side scatter) to sort EpCAM+ cells (left). The sorted cells were reanalyzed (right). c Immunofluorescence analysis of cytospin preparations of sorted P1 cells. Almost all cells were positive for proSP-C expression. Scale...
“false positive” CTCs were detected in 1 subject according to the CTC definition of DAPI+/CK+/CD45−cells (Fig.3(A)). We think that this may have been caused by the contamination of normal vascular endothelial cells during the process of venepuncture, which also reflects the current ...