骨髓中的 “播散性肿瘤细胞” ( CD31- DTC, disseminated tumor cell) 及 “播散性肿瘤血管内皮细胞” ( CD31 + DTEC, disseminated tumor endothelial cell) 在肿瘤转移过程中起着极为关键的作用,NCCN指南一直将骨髓病理活检作为部分...
骨髓中的 “播散性肿瘤细胞” ( CD31- DTC, disseminated tumor cell) 及 “播散性肿瘤血管内皮细胞” ( CD31 + DTEC, disseminated tumor endothelial cell) 在肿瘤转移过程中起着极为关键的作用,NCCN指南一直将骨髓病理活检作为部分SCLC辅助分期的重要指标,然而常规骨髓病理活检并不能有效检测肿瘤细胞及与转移密切...
Single-cell suspensions obtained from enzymatically digested murine lungs were labeled for surface antigens (CD45/CD31/epithelial cell adhesion molecule (EpCAM)/ major histocompatibility complex class II (MHCII)) and for pro-surfactant protein C (proSP-C), followed by FACS analysis for surface ...
sorted and expanded mesenchymal cells from human primary NSCLC samples based on co-expression of CD73 and CD90 while lacking hematopoietic and endothelial lineage markers (CD45, CD31, CD14 and Gly-A) and the epithelial marker EpCAM. ... CA Bichsel,L Wang,L Froment,... - 《Sci Rep》 被...
骨髓中的 “播散性肿瘤细胞” ( CD31- DTC, disseminated tumor cell) 及 “播散性肿瘤血管内皮细胞” ( CD31 + DTEC, disseminated tumor endothelial cell) 在肿瘤转移过程中起着极为关键的作用,NCCN指南一直将骨髓病理活检作为部分SCLC辅助分期的重要指标,然而常规骨髓病理活检并不能有效检测肿瘤细胞及与转移密切...
(1)在96孔酶标板中分别包被抗原ifn、ngf、cd28、cd31、csf1r、icam-1、egfr、epcam抗原完整胞外段、epcam抗原片段(以上蛋白均由北京义翘神州科技股份有限公司提供,片段由上海波泰生物公司提供),包被体积为100μl,包被浓度为0.2μg/ml),pbs作为空白对照。
将表达纯化的亲和力成熟结合蛋白用于多个无关抗原elisa,包被的抗原包括:ifn、ngf、cd28、cd31、csf1r、icam-1、egfr、epcam完整胞外段、epcam多肽。一抗换为浓度为20μg/ml的5个亲和力成熟结合蛋白(aep14e3、aep17d8、aep18e7、aep20c10、aep24d10)和野生型结合蛋白aep3d4,二抗为蛋白a-辣根过氧化物酶(protein...
A novel SE-iFISH strategy, improved for detection of HCC CTCs in this study, was applied to comprehensively detect, in situ phenotypically and karyotypically characterize hepatocellular and cholangiocarcinoma CTCs (CD45 - /CD31 - ) in patients subjected to surgical resection. Clinical significance of...