75]. Human eosinophils in anin vitrostudy have also been reported to be responsive to S100B [83], a possible blood marker in some GBM cases that is known to be released by CD8+ T cells, astrocytes, oligodendrocytes, and tumor cells [84–86]. However, the mechanisms by which...
Blood samples for RNA isolation were obtained from RA patients with different disease activity (21 females, 13 males; mean ± SD age: 61.7 ± 11.5 years; mean ± SD disease activity score 28: 4.09 ± 1.6). For high-resolution peripheral quantitative computed tomography (...
white blood cells: RBC. red blood cells. Eosinophils Table 2. S~ri~popiilu~io~tifs~ r u t i ~ r l uitc1~fi~'tu~l cut~ids udirll hloocC* Subpopulation CD 16 bright CD16 dim. with low FSC CD16 dim, with high FSC Cell type Neutrophils Eosinophils Unidentified Fetal granulocytes ...
8,29, 30, 31, 32 Whole blood cell spike-in and eosinophil clustering analyses were performed using FlowSOM and ConcensusClusterPlus algorithms from the CATALYST (github.com/HelenaLC/CATALYST) software package.28 Data analysis Relative abundance of airway cells was assessed by cytospin and CyTOF ...
The mobilization of blood leukocytes to a site of infection or inflammation in the tissues requires that the leukocytes first adhere to the endothelial lining of the blood vessel and then migrate through it. These processes involve interactions of leu- kocyte cell-surface receptors (including C D...
b, Relative expression levels of Tnfa, Il1b, Il6, p21 and Vegfa in sort-purified, blood-derived eosinophils from aged WT (20 months), young WT (3 months) or young IL5 transgenic mice (3 months) as assessed by fluidigm qPCR array. Eosinophils from 3 animals were pooled for each ...
IL-5-transgenic mice harbor at least five times more eosinophils not only in the blood as published previously17, but also in the H. pylori-infected gastric mucosa and the MLNs (Supplementary Fig. 2a–c). Eosinophils in IL-5-transgenic mice are more activated than their counterparts in ...
This supposition is supported by findings that Wnt-5a expression is increased in ASMC from asthma patients [12] and that Wnt-5a gene expression is enriched in biopsies from patients with Th2-high asthma [35]. A possible limitation of this study is that we used peripheral blood eosinophils ...
In total, 2 mg of RNA was reverse transcribed to cDNA using a High Capacity RNA to cDNA kit (Applied Biosystems, Foster City, CA). The relative expression of b-glucuronidase (Gusb), ari- ginase-1 (Arg-1), resistin-like molecule-alpha (Relm-a), and Relm-b was determined via real-...
b, Relative expression levels of Tnfa, Il1b, Il6, p21 and Vegfa in sort-purified, blood-derived eosinophils from aged WT (20 months), young WT (3 months) or young IL5 transgenic mice (3 months) as assessed by fluidigm qPCR array. Eosinophils from 3 animals were pooled for each ...