All these parameters were calculated by an automated software. Immunofluorescence staining with human synovial slides Epitopes were retrieved from deparaffinized sections using a heat-induced method. As described before19, sections were alternatively bathed in boiling citrate buffer (10 mM citric acid ...
Manual and automated eosinophil counts were compared using a Wilcoxon signed-rank test. Agreement between methods was assessed using PassingBablok and BlandAltman plots.ResultsGE were detected in all 3 breeds: 9/20 Greyhounds (45.0%), 10/29 Italian Greyhounds (34.5%), and 5/24 Whippets (...
Eosinophil and Treg numbers were positively correlated in WT mice, and Tregs were reduced—also as quantified by automated microscopy—upon eosinophil depletion (Fig. 1h, i). The phenotype of eosinophil-depleted Eo-CreDtr mice was recapitulated in PHIL mice, which transgenically express diphtheria ...
EosinophilsNeutrophilsLymphocytesMonocytesHumansLeukocyte CountPlatelet CountPregnancyPlatelets and leukocytes in venous blood were counted with automated methodology in... Pitkin,M Roy - 《Jama》 被引量: 196发表: 1979年 THE EFFECT OF PREGNANCY ON THE COURSE OF ASTHMA Bronchial asthma is a chronic disea...
The blood eosinophil count was determined with an automated hematology analyzer. Vitamin D and total immunoglobulin E (IgE) levels were evaluated using the ELISA method. Polymorphisms in the VDR gene were analyzed with real-time PCR using TaqMan probes. Results: We analyzed six VDR single ...
The peripheral blood analysis for the complete blood count test was performed on an automated hematology analyzer XE-5000™ (Sysmex, Kobe, Japan). 2.9. Granulocyte Isolation from Human Peripheral Blood and Eosinophil Enrichment Approximately 24 mL of peripheral blood was collected into sterile Ethylen...
A UniCel® DxH 800 Coulter® Cellular Analysis System automated hematology analyzer (Beckman Coulter, Miami, FL, USA) was used for the complete blood count test. Whole eosinophils’ isolation from peripheral blood procedure is described in our previous publication [16]. 2.7. Combined Cell ...
On day 30, mice were anesthetized with 2% isoflurane. Blood obtained from the aorta of mice was collected in EDTA-containing tubes. Hematological tests were performed using an automated hematology analyzer (ADVIA 2120i, Siemens Healthcare Diagnostics, Munich, Germany). ...