A collaborative approach between experiment and simulation has revealed a single mutation in the F/G loop of the newly described nitrating cytochrome P450 TxtE that controls loop dynamics and, more surprisingly, the regioselectivity of the reaction. This mutation is present in a subset of homologous...
Enzymes Transform Different Kinds of Energy In many biochemical reactions, the energy of the reactants is converted into a different form with high efficiency. For example, in photosynthesis, light energy is converted into chemical-bond energy. In mitochondria, the free energy contained in small ...
RNA can undergo a variety of modifications, but only a small proportion of modifications lead to changes in RNA base pairing, that is, RNA editing [1,2,3]. The discovery of RNA editing began with studies of the biochemical reactions of adenosine deamination and recoding events in the mammalia...
assay. Reaction rates were determined by fitting progress curves using linear regression and are expressed as initial turnover rates (µM/s). Controls contained fecal extract that had been heat-killed at 95 °C for 5 min. Reported rates are the average of three biological replicates ±...
as they increase the reaction rate by many orders of magnitude. Until recently, enzymes (and proteins in general) were considered static assemblies; however, recent investigations continue to provide evidence which indicate that enzymes are dynamically active assemblies. Detailed experimental and theoretica...
and hence to increase reaction rates Raising the reaction temperature -- To increase the average energy of reactants -- Reaction rates are usually doubled by a 10oC rise in temperature Adding catalysts -- To lower the energy of the transition state -- Regenerated after each reaction cycle ?
Y Degani,C Degani - 《Trends in Biochemical Sciences》 被引量: 23发表: 1980年 Absence of kinetic negative co-operativity in the allosteric model of Monod, Wyman and Changeux An equation is derived for the Hill coefficient n H based on the relationship between reaction velocity and substrate ...
which is optimally active at 90°C and pH 5.5–6.0. This enzyme also formed less isomaltose, a common side reaction, than the commercially available fungal glucoamylase [85]. To increase the efficiency in saccharification, a debranching enzyme, such as pullulanase, can be added to the process...
A better understanding of the effects of spatial confinement on protein function will not only enhance our fundamental knowledge of cellular organization and metabolism but also increase our ability to translate biochemical pathways into a variety of noncellular applications, ranging from diagnostics and ...
Tropical legumes transport fixed nitrogen in form of ureides (allantoin and allantoate) over long distances from the nodules to the shoot. Ureides are formed in nodules from purine mononucleotides by a partially unknown reaction network that involves bac