Enzyme promiscuity of carbohydrate active enzymes and their applications in biocatalysisThe application of biocatalysis for the synthesis of glycans and glycoconjugates is a well-established and successful stra
In consequence, there has been much interest towards the synthesis of HMOs industrially, for use as probiotics, for which a number of approaches have been used, including direct chemical syntheses, use of recombinant glycosyltransferase (GT) activities34; transglucosidase reactions, in which glycohydro...
Lysosomal metabolism of glycoconjugates. Subcell Biochem 27: 191–238. 8. Bame, KJ (2001). Heparanases: endoglycosidases that degrade heparan sulfate proteoglycans. Glycobiology 11: 91R–98R. 9. Stanford, KI, Bishop, JR, Niesman, IR, Witztum, JL and Esko, JD (2009). Syndecan-1 is ...
Carbohydrate-active enzymes (CAZymes) are involved in the metabolism of glycoconjugates, oligosaccharides, and polysaccharides and, in the case of plant pathogens, in the degradation of the host cell wall and storage compounds. We performed an in silico analysis of CAZymes predicted from the genomes...
(1996). Enhancement of wine aromas by enzyme hydrolysis of glycosidic precursors from the grape. In C.S. Stockley, A.N. Sas, R.S. Johnstone & Lee, T.H., (Eds.),Maintaining the competitive edge: proceedings of the ninth Australian wine industry technical conference. Adelaide, South ...
Cell-free extracts from the conidia produce ATP in the presence of AMP and phosphoenolpyruvate. The system is Mg2+-dependent and highly stimulated by increases in the viscosity of the incubation media. The examination of enzymes involved in the ATP synthesis revealed the presence of adenylate ...
Human milk oligosaccharides (HMOs) are a crucial ingredient in mother’s milk for infant health. These are a structurally diverse group of soluble bioactive glycans having antiviral and anti-bacterial properties and promote the growth of probiotics, particularlyBifidobacteria. They modulate the immune sy...
Although the acidic hydrolysis during wine ageing can transform them into free volatile aroma compounds, a slow process and faster enzymatic hydrolysis of these glycoconjugates are performed by wine microorganisms. Thus, the liberation of the odorous aglycon is achieved by the action of the β-D-...
The oligosaccharide structures of glycoconjugates are synthesized in vivo by Leloir glycosyltransferases which require activated sugars (nucleotide- sugars) as donor substrates for the regio- and stereoselective transfer. The nucleotide-sugars are produced by nucleotidyltransferases or pyrophosphorylases (for...
The low cost of sucrose and lactose, combined with the broad acceptor substrate acceptance of glucansucrase enzymes, makes them useful tools in the synthesis of novel and potentially prebiotic oligosaccharides. This study explored the ability of glucansucrase enzymes Gtf180-ΔN and GtfA-ΔN from ...