For most proteins annotated as enzymes, it is unknown which primary and/or secondary reactions they catalyze. Experimental characterizations of potential substrates are time-consuming and costly. Machine learning predictions could provide an efficient al
The turnover numberkcat, a measure of enzyme efficiency, is central to understanding cellular physiology and resource allocation. As experimentalkcatestimates are unavailable for the vast majority of enzymatic reactions, the development of accurate computational prediction methods is highly desirable. However...
so they are being improved in different ways. For example, the short half-lives of gRNAs used in RNA editing tools (especially those without protein binding) limit the efficiency and sustainability
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Therefore, the examination of digestive physiology is of major concern to evaluate the whole digestive process efficiency mostly depends upon the type and function of the digestive enzymes. The comparative studies of these enzymes and their activities in different parts of the digestive system and in...
we have been mostly dependent on material that is not publicly peer-reviewed (naturally regulators have had access to original studies), but on the other hand, studies providing the basis for official drug monographs are expected to be of high quality. Furthermore, many of them have appeared ...
They are mostly electrophiles, and all can react chemically with thiol groups by alkylation, oxidation, or reduction, suggesting to us that the intracellular sensor for inducers is likely to contain very highly reactive (cysteine) thiols. This suggestion was bolstered by the following: (a) the ...
we have extended our single-enzyme analysis of cytochrome bo3 and show that (a) increased membrane curvature reduces proton pumping activity; (b) leak states might be retained by cytochrome bo3 directly after turn-over has been halted and; (c) proton transport activity is mostly affected by the...
(PtLDC,PtCAO andPtPIKS) (Extended Data Fig.3j,k). Also, we observed consumption of both8and3that was concurrent with production of9, which supports these compounds as the ‘pseudodimers’ that are condensed to form the scaffold molecule (Fig.2and Extended Data Fig.3l). BecausePtPIKS ...
Structure of XacGH43_1 without sugar (a), where a glycerol molecule occupies the -1 subsite; in complex with xylose (b) and in complex with xylotriose (c). In all structures, the main interactions in the −1 subsite and with Ca2+ (green sphere) are shown with distances in angstroms...