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The differences in enzyme properties of the single-chain Eco94GmrSD and two-chain GmrS/GmrD complex. The major differences of the single-chain Eco94GmrSD and the two-chain GmrS/GmrD are: 1) Ca21 is not required for Eco94GmrSD activity, (Ca21 and Mg21 required for the two-chain ...
This would help to explain the change in enzyme specificity by a single amino acid substitution in Fuc-TIII, whereas more complex changes are expected in Fuc-Tb or Fuc-TVI to generate a strong type 1 acceptor activity because other independent mutations might have occurred in Fuc-TVI and/or ...