By using the kit provided by the invention, infected patients with hand-foot-and-mouth diseases can be subjected to EV71 detection quickly and accurately, so that a new way is provided for the diagnosis and prevention of EV71 virus infection, therefore, the enzyme-linked reaction detection kit ...
In this study, we developed a flexible genotyping method involving fill-in ligation reaction with enzyme-linked immunosorbent assay successfully applied to detect important single-nucleotide polymorphisms (SNPs) for EGFR c.2573T > G (L858R), EGFR c.2582T > A (L861Q), and EGFR c.2155G > T...
enzyme-linked-immunosorbent serologic assay - an assay that relies on an enzymatic conversion reaction and is used to detect the presence of specific substances (such as enzymes or viruses or antibodies or bacteria) ELISA assay - a quantitative or qualitative test of a substance (especially an ore...
HRP can be measured with a detection limit of 6.0×10-(10) g/L and a linear range of 1.0×10-9—4.0×10-6 g/L. The pure product of H2O2 oxidizing OAP catalyzed by HRP was prepared with chemical method. The enzyme-catalyzed reaction has been investigated with electroanalytical chemistry,...
and exposed either to a complex of an enzyme linked to an antibody specific for the antigen or an enzyme linked to an anti-immunoglobulin specific for the antibody followed by reaction of the enzyme with a substrate to yield a colored product corresponding to the concentration of the test ...
Reid SM,Forsyth MA,Hutchings GH, et al.Comparison of reverse transcription polymer- ase chain reaction, enzyme linked immunosorbent assay and virus isolation for the routine diagnosis of foot-and-mouth disease. J Virol Meth . 1998Reid, SM, Forsyth, MA, Hutchings, GH, Ferris, NP ...
Enzyme-linked immunosorbent assay(ELISA) was first described in 1971. It consists of a two-pronged strategy: (1) the reaction between the antibody and its corresponding antigen and (2) the detection of that reaction using an enzyme, labeled to the reactants, as an indicator. This technique ...
viachemiluminescentreactionwithHRP.Nomatterwhichyou choose,KPL’sELISAsubstratesarealltestedtoassurelot-to-lot consistency,reproducibilityandlong-termstability. Anextensivelineofhigh-qualitysecondaryantibodyconjugatesand labeledstreptavidinarealsoavailable.Eachisaffinitypurifiedto ...
(Sofroniew, 2005; Yuan and He, 2013). The reaction of miR-21 in astrocytes following SCI is regulated bybone morphogenetic protein(BMP) signaling pathway (Sahni et al., 2010). The signaling through BMPR1a reduced the miR-21 expression while the signaling through BMPR1b increased miR-21 ...
reaction were denatured with 20 ,ul of denaturation solution and filled up to 250 ,ul with hybridization solution containing the biotinylated hybridization probe (7.5 pmol/ml). Hybridization was done using different incubation ...