Suggest one method that could have been used to keep the tubes at a constant temperature. [1 mark] Explain the importance of having equal quantities of the enzyme at the start of the experiment. [2 marks] 3. Explain the production of lactose-free milk. [3 marks] 4. The diagram below ...
The use of the classic Henry–Michaelis–Menten (HMM) model (or simply, Michaelis–Menten model) to study the substrate and enzyme concentration dependence of enzyme catalysis is a very important step in understanding many biochemical processes, including microbial growth. Although the HMM model has...
The concentration of substrates of enzyme reactions is measured by determining the current produced at a platinum or gold electrode by oxidizing an acceptor utilizing a measuring device comprising a measuring cell, an enzyme electrode in contact with the fluid sample and containing a platinum or gold...
SeeFigure 2for a comparison of the range of sensitivities for the different formulations and selection guide (Tab 1) for the optimal kit for your experiment. ABTS ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt)is used to detect HRP an...
reaction increases with increased substrate concentration, reaching maximum velocity when all active sites of the enzyme molecules are engaged. The enzyme is then said to be saturated, the rate of the reaction being determined by the speed at which the active sites can convert substrate to product...
When passive cell loading or enhanced dye retention are critical parameters of the experiment, we recommend using our lipophilic substrates for glycosidases (such as our ImaGene Green and ImaGene Red products, Detecting Glycosidases—Section 10.2). Like resazurin ...
The effect of spraying different concentration of Se on lettuce's SOD, POD, MDA and net photosynthesis speed on the pot experiment was studied in the paper... H Du,L Feng,Z Niu,... - 《Chinese Agricultural Science Bulletin》 被引量: 22发表: 2007年 Effects of a soluble fiber with exce...
5). Upon substrate recognition, TRIM21 forms a K63-linked ubiquitin chain on its N-terminus13. Loss of this K63-linked ubiquitin chain prevents virus neutralization, immune signaling, and Trim-Away17. Our GFP-Fc degradation experiment shows that only the TRIM21 construct (R-R-PS) that can...
Yueyao Wang performed the in vitro selection, biochemical characterization, structural probing and substrate selectivity experiments. Yao Wang performed the intracellular gene silencing experiment. D.S. analysed Tz1 and cleavage reaction products by mass spectrometry. X.S. analysed the biological stability...
heatstroke and collapse occurred after about 2 hours of exposure (Tre−44.1°C), together with mean dehydration of 6.1%(Table 2).Significant rise in blood enzyme levels started two hours later and achieved peak values at 350 minutes. Four out of five dogs survived the experiment. Their bloo...