doi:10.1016/0022-5193(81)90323-4GeorgeH.WeissandIvanG.DarveyElsevier LtdJournal of Theoretical BiologyWeiss GH, Darvey IG. A note on the choice of substrate concentration in enzyme kinetic experiments. J Theor Biol. 1981 Jun 7; 90 (3):437–439.
2.5.S1 Design of experiments to test the effect of temperature, pH, and substrate concentration on the activity of enzymes. 2.5.S2 Experimental investigation of a factor affecting enzyme activity. 酶反应有最适合的pH、温度等条件,而这些条件也正好是身体稳态(homeostasis)维持所必须的。 温度影响的实验设...
See Figure 2 for a comparison of the range of sensitivities for the different formulations and selection guide (Tab 1) for the optimal kit for your experiment. ABTS ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is used to detect H...
The use of the classic Henry–Michaelis–Menten (HMM) model (or simply, Michaelis–Menten model) to study the substrate and enzyme concentration dependence of enzyme catalysis is a very important step in understanding many biochemical processes, including microbial growth. Although the HMM model has...
SeeFigure 2for a comparison of the range of sensitivities for the different formulations and selection guide (Tab 1) for the optimal kit for your experiment. ABTS ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt)is used to detect HRP an...
Free Essays from Studymode | The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment...
To ensure maximum enzyme concentration, the immobilization experiment was scaled up fourfold. The antibodies used were (1) purified CHO h-IgG, (2) h-IgG (Sigma-Aldrich; resuspended in deionized water and stored at 4 °C) and (3) r-IgG (Sigma-Aldrich; resuspended in deionized water and...
Yueyao Wang performed the in vitro selection, biochemical characterization, structural probing and substrate selectivity experiments. Yao Wang performed the intracellular gene silencing experiment. D.S. analysed Tz1 and cleavage reaction products by mass spectrometry. X.S. analysed the biological stability...
An early experiment showed that immobilized papain (a protease derived from papaya latex) retained over half its room-temperature activity at 80°C in conditions under which the soluble enzyme was almost entirely denatured. Also interesting and valuable are the changes in the pH-activity curves ...
An enzyme will interact with only one type of substance or group of substances, called thesubstrate, to catalyze a certain kind of reaction. Because of this specificity, enzymes often have been named by adding the suffix “-ase” to the substrate’s name (as inurease, which catalyzes the ...