Using NMR spectroscopy to elucidate the role of molecular motions in enzyme functiondoi:10.1016/j.pnmrs.2015.11.001George LisiJ Patrick Loria
Compensatory and Long-Range Changes in Picosecond–Nanosecond Main-Chain Dynamics upon Complex Formation: 15N Relaxation Analysis of the Free and Bound Sta... Mapping of the Rac1 GTPase surface that contacts the Rho GTPase binding domain of plexin-B1 by solution NMR spectroscopy confirms the plexin...
The force dependence provides valuable new information about the dynamics of enzyme catalysis with sub-angstrom resolution, a feat unmatched by any other current technique. To date, single-molecule force spectroscopy has been applied to gain insight into the reduction of disulfide bonds by different ...
coherently due to their pronounced disorder. The conformational variations of loop regions become evident in NMR structural ensembles, which show a multitude of different conformations for the same loop. The high mobility of loops has been frequently confirmed by trajectories of molecular dynamics ...
et al. Coherent multidimensional optical probes for electron correlations and exciton dynamics: from NMR to X-rays. Acc. Chem. Res. 42, 553–562 (2009). A review of multidimensional spectroscopy techniques with an outlook to future XFEL applications. Article MathSciNet Google Scholar Schweigert...
Understanding the role of conformational dynamics in enzyme evolution is a relatively young field. Despite this, there have already been several seminal pieces of work from either computational or an experimental perspective, and, as has been discussed by both ourselves and others [48•,49••...
NMR Spectroscopy. NMR spectra were acquired at 298 K on a Bruker DRX 700 MHz spectrometer operat- ing at the proton frequencies of 700.13 MHz (16.44 T) on uniformly 15N- enriched samples of ca. 0.5 mM UreG in 20 mM TrisHCl buffer at pH 8, containing 150 mM ...
. The findings have been possible through an integrative structural biology approach where we utilize state-of-the-art quantitative 19F NMR spectroscopy (relaxation dispersions) for determination of microscopic rate-constants for conformational dynamics of a large (69 kDa) assembly....
31P NMR spectroscopy is thus an important probe of structure, dynamics and mechanism in native and site-directed mutations of PLP-dependent enzymes. Examples of all of the above are provided in this review. This article is part of a Special Issue entitled: Pyridoxal Phospate Enzymology....
Further investigation of the relative B factor profile and molecular dynamics simulation confirmed that the enhanced rigidity decreased fluctuation of the active site residues at high temperature. These results indicate that enhancing the rigidity of the flexible segment within the active site may provide...