Define Restriction enzymes. Restriction enzymes synonyms, Restriction enzymes pronunciation, Restriction enzymes translation, English dictionary definition of Restriction enzymes. n. Any of a group of enzymes that catalyze the cleavage of DNA at specific
The reaction could be controlled by judicious choice of the solvent. 3-Hydroxy-oxindoles and 3,3-bis(indol-3-yl)indolin-2-ones were obtained in aprotic (DCE) and protic solvent (MeOH), respectively; the reactions proceeded at 30掳C with a broad substrate scope in each case. Several ...
Because this method relies on a weak interaction between the enzyme and the support, the biocatalyst can easily desorb during the reaction due to changes in the conditions of the reaction media (pH, ionic strength, temperature, pressure, etc.). However, the weak interactions generally do not ...
Such a coupled reaction system works very well in vitro [112]. Although, oxime-mediated reactivation of BChE is slow compared to reactivation of phosphylated AChE [113,114], considerable progress were accomplished in the past years, and the pseudocatalytic bioscavenger approach based on the ...
different pH values, and the consumption ratio ofl-glutamate between RocG and GlnA was 2:1 at pH 8.0, representing the practical regeneration ratio of these two enzymes in simulative AdoCbl synthesizing system that formed of 2 mMl-glutamate, 1 mM NAD+and 1 mM ammonium (Supplementary Fig...
The controlled interactions with key cofactors and reaction intermediates make this technology even more promising for a wide range of applications in biocatalysis. Furthermore, the applications demonstrated in this work could be expanded to other fields of applied science, for instance, the integration...
An enzyme reaction stabilizer consisting of poly-L-lysine or its salt as an effective constituent, a method for using the enzyme reaction stabilizer, and an enzyme preservative consisting of poly-L- lysine or its salt as an effective con... T Shinto,J Hiraki - US 被引量: 5发表: 1997年...
Five sera specific IgE of all allergic subjects showed reaction with only C. arizonica pollen extracts. Conclusions: As a conclusion, the pollen structure, protein function or protein structure and isoforms of allergens could affects allergenic properties of the pollen. This study also may help to ...
It was not until the spread of antibiotics that the trend of bacterial infections was effectively controlled. However, the overuse and misuse of antibiotics has led to the emergence of drug-resistant strains of bacteria, posing a great threat to public health and the environment [2]. It is ...
When [S] is equal toKm, the rate of reaction is equal to half the maximal velocity, by replacing, Eq.(8.1)becomes: v=Vmax[S]Km+[S]=Vmax[S][S]+[S]=Vmax[S]2[S]=Vmax2 The Michaelis–Menten equation can be transformed algebraically into equivalent equations useful for practical determ...