This book provides a comprehensive overview of the recent developments achieved in the field of chemo/enzymatic cascades with topics spanning from design (in vitro and in vivo) to kinetic- and process modelling as well as process control. Opportunities and challenges of building multi-step chemo/en...
Now, a mechanism for O2 diffusion within FeFe hydrogenases and its reactions at the active site of the enzyme has been proposed. These findings could help with the design of hydrogenase mutants with increased resistance to oxidative damage. Adam Kubas , Christophe Orain & Christophe Léger ...
In this study, a four-enzyme cascade pathway was developed and reconstructed in vivo for the production ofd-p-hydroxyphenylglycine (D-HPG), a valuable intermediate used to produce β-lactam antibiotics and in fine-chemical synthesis, froml-tyrosine. In this pathway, catalytic conversion of the i...
Toward this aim, we propose the use of engineered proteins to assemble multi-enzyme systems, encoding by design the nanometric proximity of catalytic domains and the binding sites to reversibly sequester cascade intermediates. Although widely used for the fabrication of genetically programmed biomaterials...
If the model is mechanistic, it has the power to both infer molecular mechanisms and predict biological responses [2]. This requires the simulation of biochemical reaction kinetics typically described using ordinary differential equations (ODEs). Modelling enzymatic cascade networks, however, requires ...
distance on fusion proteins. Therefore, without causing the metabolic burden of host cells, the design of "quantitative, sequential and spatial" controllable multi-enzyme cascade reaction is a hot topic for international academic circles. Some researchers have been trying to design fusion proteins ...
to the type 2 enzyme described later, which converts estradiol to the less potent estrogen estrone. The crystal structure of the type 1 enzyme has been determined to a resolution of 2.2 Å with and without bound substrates, providing a molecular framework for the design of specific inhibitors...
Understanding the practical half-life and stability of any free-, substrate-bound, or surface-immobilized enzyme can be critical to the design of proper biocatalysis protocols. Considerations include: Solvents—Many natural enzymes struggle to cope with organic solvent reactions. A constantly evolving pr...
(iii)Reusability and stability. Another important challenge is to design immobilized enzymes that are stable and reusable over a long period of time. The immobilization process and the support material used should not affect the stability of the enzyme and should allow it to be used for multiple...
In the latter design the cells are entrapped between the space formed between the outside of the hollow fibre and the flat sheet membrane. The medium flows through the lumen of the fibres while oxygen and carbon dioxide are supplied through the hydrophobic flat sheet membrane. The final ...