酶的特异性(Enzyme specificity)及活性位点的诱导契合模型(induced-fit model) 与工业化中用到的无机催化剂不同,酶在发挥作用时有高度的专一性。酶的活性位点中的氨基酸排布方式刚好使得能其与底物的某些化学基团发生结合,形成enzyme–substrate complex。 在enzyme–substrate complex形成之后,酶的活性位点结构会发生稍微...
1. Enzyme as biological catalyst2. Enzymes can be used repeatedly (酶可以重复使用)3. A small amount of enzyme is required4. Enzyme is a kind of protein5. affected by temperature and pH value of the environment. (酶会被温度,pH等影响)6...
Bradbury et al., Substrate Specificity Of An Amidating Enzyme in Porcine Pituitary, Biochemical and Biophysical Research Communications, vol. 112, No. 2, 1983, pp. 372-377.Bradbury AF, Smyth DG. Substrate-specificity of an amidating enzyme in porcine pituitary. Biochem Biophys Res Comm 1983;112...
We conclude that evolutionary and structural information may be combined on a Structural Genomics scale to create motifs of mixed catalytic and noncatalytic residues that identify enzyme activity and substrate specificity. 展开 关键词: 3D COORDINATE TEMPLATES FUNCTION ANNOTATION THERMOSTABLE ESTERASE ...
Dunn BM, Hung S (2000) The two sides of enzyme-substrate specificity: lessons from the aspartic proteinases. Biochim Biophys Acta 1477:231–240Dunn, B. M., and S.-H. Hung. 2000. The two sides of enzyme-substrate specificity: Lessons from the aspartic proteinases. Biochim. Bio- phys. ...
In subject area: Biochemistry, Genetics and Molecular Biology Substrate specificity engineering is not intended to increase the catalytic turnover of a substrate, but exploited to promote the reaction of one substrate over another by governing accessibility to the active site of an enzyme. ...
Human APOBEC3A is a single-stranded DNA cytidine deaminase that restricts viral pathogens and endogenous retrotransposons, and has a role in the innate immune response. Furthermore, its potential to act as a genomic DNA mutator has implications for a rol
A.Binding energy provides the enzyme specificity for the substrate.B.Binding energy contributions allow for entropy reduction in the substrate-enzyme complex.C.Binding energy compensates for energy changes as a result of desolvation of the substrate.D.Binding energy contributes to the process of induc...
Berger, and Jack F. Kirsch - 《Biochemistry》 被引量: 51发表: 2004年 Narrowing substrate specificity in a directly evolved enzyme: The A293D mutant of aspartate aminotransferase Kirsch, Narrowing substrate specificity in a directly evolved enzyme: the A293D mutant of aspartate aminotransferase, ...
Despite the immense importance of enzyme–substrate reactions, there is a lack of general and unbiased tools for identifying and prioritizing substrate proteins that are modified by the enzyme on the structural level. Here we describe a high-throughput u