Temperature Effects in Enzyme ActivityThe Effects of Temperature
因为是研究者主动改变温度来观察对酶活性(因变量)的影响。选项A结果和选项B成果在这里不符合变量的概念;选项D因变量是受自变量影响而变化的,这里温度是自变量不是因变量。本题考查实验变量概念,词汇有“enzyme activity(酶活性)”,语法为简单的主系表结构。
Enzyme activity is temperature dependent, increasing with rising temperature, reaching a maximum activity (known as optimum temperature), before it starts to decrease (Fig. 34.2B). The optimum temperature is different for each enzyme. Nevertheless, enzymes are proteins, and temperature excess (>60°...
This lesson addresses how temperature influences enzyme function, and provides a concrete example from a lab setting. The key terminology will...
Enzyme activityis routinely measured by following the reduction of NAD+at 340nm with a cocktail consisting of 30mMsodium pyrophosphate, pH8.0, adjusted with HCl at room temperature, 2mMDTT, 2mMNAD+, 0.5mMCoA, and 0.5mMmalonate semialdehyde or methylmalonate semialdehyde. Reactions are initiated with...
The effect of pH and temperature on enzyme activity can also interact with each other. Enzymes may have different optimal temperature ranges depending on the pH of the environment. For example, acid phosphatase, an enzyme found in the prostate gland, has an optimal temperature range of 30-40°...
An enzyme exhibits maximum activity over the narrow pH range in which a molecule exists in its properly charged form. The median value of this pH range is called theoptimum pHof the enzyme (part (b) ofFigure 18.14 “Temperature and pH versus Concentration”). With the notable exception of ...
has been checked ,due to increase of 10℃ it is seen that the enzyme activity has been increased by 50-60% . Example- Optimum temperature of the maximum digestive enzymes in our body is 37℃ , according to our own body temperature. Enzymes activity decreases due to decrease of temperature...
Some proteins can regain their shape and function after denaturation if the denaturing agent is removed, and the presence of protein re-folding enzymes called molecular chaperones helps. However proteins denatured by heat do not renature, and heat is a reliable way to "kill" the activity of an...
Optimization to low temperature activity in psychrophilic enzymes. Int. J. Mol. Sci. 13, 11643–11665 (2012). Article CAS PubMed PubMed Central Google Scholar Harms, M. J. & Thornton, J. W. Evolutionary biochemistry: revealing the historical and physical causes of protein properties. Nat....