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This pattern is also observed when we measured β-lactamase activity using the chromogenic β-lactam derivative nitrocefin (Fig. 6D). Knockdown of penB reduced β-lactamase activity by ~90% and was further inhibited to ~98% when AVI was added. There was no effect when K562_RS32470 was ...
The distribution of LukAB on bacterial cells exhibits a punctate pattern. Previously, several proteins have been characterized to be discretely localized in the membrane of S. aureus, including the functional membrane microdomain FloA and its cargo proteins, as well as the lipid synthesis proteins ...
This same pattern is found when the PCT64-35B Fab was produced in the absence of TPST1, suggesting a high level of endogenous sulfation of the relevant tyrosine. The residual difference between Fab in the minority population and the theoretical mass of the Fab fragment (∼245 Da) is ...
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2c), a pattern similarly observed in the secretion of mIL33 and mIL36α (Supplementary Fig. 2a, b). Fig. 2: E-regulated UcPS is dependent on TMED10. a Immunofluorescence of U2OS expressing GFP-ERGIC53 and Myc-tagged E of indicated coronaviruses. b Immunofluorescence of U2OS expressing ...