htlv-1env属于i型跨膜蛋白,以488个氨基酸的前体蛋白形式于糙面内质网中合成、折叠并伴随着寡聚化和初始糖基化,随后被输送到高尔基体中,经furin样蛋白酶家族剪切形成成熟的表面糖蛋白gp46和跨膜糖蛋白gp21,酶切后gp46和gp21仍非共价地联系在一起形成异二聚体,最后形成由三个gp46分子和三个gp21分子构成的三聚体...
The Env protein is then exported to the Golgi, and cleaved by furin proteases to form the separate SU and TM subunits. This cleavage is essential for the normal function of the Env protein. The cleavage occurs at a dibasic pair of amino acids (257), and produces a hydrophobic Nterminus ...
To mitigate the need for sufficient furin expression in vivo, cleavage-independent designs were created with a 2x[G4S] linker56,57 (Supplementary Fig. 5a). The ConC, sC4 and C97ZA Env encoding Ad26 vector particles were mixed in a 1:1:1 ratio to generate a trivalent cocktail termed Ad...
His-tagged 426c gp120 was engineered by disrupting the 426c furin cleavage site (RNKR →RNKG) followed immediately by the addition of a 7x-polyhisitidine tag and stop codon (forward gp120-His mutagenesis primer, 5′- ggaacaagggcgctcatcatcaccaccatcaccattgataggtggggatcggagc -3′). Recombi...
This simplified design avoids the need for furin co-expression and, in one case, antibody affinity purification to accelerate trimer scale-up for preclinical and clinical applications. We have successfully translated the NFL design to multiple HIV-1 subtypes, establishing the potential to become a ...
其中gag编码p71的前体蛋白,病毒编码的蛋白酶对其C端加工后产生一个p68的成熟GAG蛋白;GAG蛋白能够刺激机体产生体液和细胞免疫.HFV的env基因编码gp130的前体蛋白,它被细胞furin样蛋白酶切割成gp18的前导肽蛋白(LP)、gp80-85的外膜蛋白(SU)和 gp45-48的穿膜蛋白(TM)[3];外膜蛋白是 HFV 的主要抗原,同时是中和...
综上所述,本课题组认为宿主限制因子BST-2除了文献报道的经典抗病毒机制以外,还可以通过减少Furin表达水平,抑制Furin成熟来降低Furin酶活力.与此同时,Furin加工HIV-1 Env的功能会被BST-2抑制,BST-2还能与Furin底物gp160相结合.因此Env成熟体gp120,gp41在病毒颗粒中的包装将受到影响,最终这将使HIV-1病毒颗粒的感染...
Inhibition of furin-mediated cleavage activation of HIV-1 glycoprotein gp160. Comments on how the envelope glycoprotein of human immunodeficiency virus (HIV) initiates infection by mediating fusion of the viral envelope with the cell... Hallenberger,Sabine,Bosch,... - 《Nature》 被引量: 675发表...
an ordered 16055 sequence (Genbank: EF117268.1 [https://www.ncbi.nlm.nih.gov/nuccore/EF117268.1]; Integrated DNA Tech- nologies) that had the furin cleavage site (REKR) substituted for RRRRRR and the following mutations introduced: H66R, A73C, T316W, A501C, I559P, A561C, and T605C....
Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield. Here, we show that a cleavage-independent...