SufianK N,Hira T,Nakamori T,et al.Soybean (3 - conglycinin bro-melain hydrolysate stimulates cholecystokinin secretion by enteroen-docrine STC - 1 cells to suppress the appetite of rats under meal -feeding conditions [J]. Biosci Biotechnol Biochem, 2011 ; 75 ( 5 ):848 -853....
The results presented here show that STC-1 cells, a model of intestinal endocrine cells, respond to a broad range of amino acids, including l-proline, l-serine, l-alanine, l-methionine, l-glycine, l-histidine, and alpha-methyl-amino-isobutyric acid (MeAIB) with a rapid increase in the...
Here, we show that murine mucosal homogenates and STC-1 cells, a murine enteroendocrine cell line, express mRNA for all l-AA receptors. Immunohistochemical analysis demonstrated the presence of all l-AA receptors on STC-1 with CaSR being most commonly expressed and T1R1 least expressed (35% ...
Enteroendocrine K cells have characteristics similar to pancreatic β-cells, such as a glucose-sensing system and insulin-processing proteases. In this study, we genetically engineered an enteroendocrine cell line (STC-1) to express insulin under the control of the glucose-dependent insulinotropic ...
To our knowledge, this is the first report to study the stimulation of CCK peptide hormone secretion from STC-1 cells by quercetin and kaempferol, rutin, apigenin and baicalein. Based on the cell-based results in this work, it can be suggested that the reported activity of flavonoids against...
In this study, we show that bile acids promote glucagon-like peptide-1 (GLP-1) secretion through TGR5 in a murine enteroendocrine cell line STC-1. In STC-1 cells, bile acids promoted GLP-1 secretion in a dose-dependent manner. As STC-1 cells express TGR5 mRNA, we examined whether bile...
Although all gastric and intestinal digests from both substrates induced the secretion of cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1) in STC-1 cells, GLP-1 levels were maximum in response to gastrointestinal digests from the hydrolysate. The enrichment of protein ingredients with ...
Objective: To deepen on the knowledge of the mechanisms used by GSPE to increase GLP-1, and extend it to its role at modulation of other enterohormones. Design: We used anex vivosystem to test direct modulation of enterohormones; STC-1 cells to test pure phenolic compounds; and rats to ...
Here, we examined the effect of zinc on CCK secretion in STC-1cells, an enteroendocrine cell line derived from murine duodenum, and in rats. Extracellular zinc triggered CCK secretion accompanied with increased intracellular Ca(2+) and Zn(2+) mobilization in STC-1cells. Zinc-induced CCK ...
Cells were incubated overnight in 96-well black/clear plates (CORNING Inc., Kennebunk, ME; HuTu-80, 7.5 × 104 cells/well; STC-1, 5 × 104 cells/well) in minimum essential medium α (HuTu-80) or Dulbecco’s modified Eagle medium/F-12 (STC-1) without phenol red (Gibco). [Ca2+...