COMELEC will use the Gemalto solution to verify the identity of voters. This was put to a test at the first time during the mock elections held on 19th Jan 2019, in preparation for the actual polls in May 2019. Instead of manually checking the COMELEC’s voters list, members...
The column was then pre-equilibrated in buffer A (6 μl) before being connected in-line to an Eksigent NanoLC-Ultra 2D plus HPLC system (AB-SCIEX, Concord, ON) coupled to a Thermo Electron LTQ-Orbitrap Velos (Thermo Scientific, Mississauga, ON) equipped with a Proxeon Biosystems nano...
For the screen, an siRNA library consisting of a mixture of 4 siRNAs corresponding to 1280 kinases, phosphatases, metalloproteases, and G protein-coupled receptors and associated proteins of the human proteome was used (Dharmacon ON-TARGETplus®SMARTpool®siRNA Library, Human Druggable Subsets, G...
The labeling of cells progressing though S-phase was performed by either intraperitoneal injections of EdU (20 mg/kg) or through administration of EdU in the drinking water (0.2 mg/ml). For long-term EdU water administration, fresh solution was replaced at least every 56 hours. The sensitivity...
(BL21-CodonPlus) was induced by the addition of 0.1 mM isopropyl β-d-1-thiogalactopyranoside to media followed by incubation for 12 h at 20 °C. For purification of GST-tagged proteins, cells were collected and resuspended in lysis buffer (20 mM HEPES-KOH pH 7.6, 0.5 M ...
In some experiments, to remove cell surface of heparan and chondroitin sulfates, HEC-1 cells were pretreated 1 h at 37 °C in Hank's balanced salt solution with heparinase III (5 U/ml from Sigma), phospholipase C (5 U from Sigma) or chondroitinase ABC (10 U from Sigma), as ...
In Video S2, for cells in which the zero points did not converge into a solution, we depicted 5×5 pixels square at the location of their nuclei. The border localized at the edge of the cytoplasm was further enlarged by Dilate (3D) functions until it overlapped the cell membrane. This ...
The proteins were eluted in 1 ml of Biotin solution (2mg/ml; Sigma) for 4 hours at 4OC followed by affinity pull-down with S-protein coupled to agarose beads (Novagen) for 4 hours at 4OC. SFB tagged GFP was used as a control for the pull-down experiments. Following pull-down, ...
One volume of the cell suspension was added to one volume of a 4x dye solution (4 μM JC-1, 40 μg/ml oligomycin, 0.02% digitonin, 20 mM 2-mercaptoethanol in DTEB). The cell/dye solution was left at room temperature for 10 min to allow permeabilisation and dye equilibration. The...