Dietary gelatin thus seems to enhance non-heme iron absorption via regulating systemic iron homeostasis rather than via solubilizing luminal ferric iron.Wu, LingyuZou, YaqunMiao, YuZhang, JiayouZhu, SuqinZeng, MingyongWu, HaohaoOcean Univ China Coll Food Sci &Journal of Functional Foods...
The activity of Catalase (CAT) was assessed by monitoring the H2O2breakdown in the reaction mixture [27]. The 2 mL of reactant was comprised of H2O2(30 mM), sodium phosphate buffer (50 mM), and 0.2 mL of crude enzyme extract. The absorption of the reaction mixture was observed by a U...
TABLE 1. Absorption maxima for P. pantotrophus cytochrome cd1 and its complexes referred to in this work Unless stated, data are for d1 heme absorption bands, were obtained at pH 7.0, and are for wild type enzyme. Rows i and ii refer to oxidized “as isolated” cytochrome cd1 and fully...
This reaction generates a yellow derivative (TNB) whose absorption is measured spectrophotometrically at 412 nm. Briefly, 30 µL of 10 mM DTNB were added to a medium containing 490 µL of PBS and 50 µL of tissue supernatants (approximately 50 μg of protein). The medium was then ...
dietary supplementations with gelatin and its two major degradation products, glycine and prolyl-hydroxyproline, revealed that gelatin boosted iron absorption not through glycine, but rather via prolyl-hydroxyproline, which had inhibitory and stimulatory effects on the plasma levels of hepcidin and eryt...
Meat enhances nonheme Fe absorption from most diets. It is not known whether these effects also occur in the pig, a promising model for human Fe absorption studies. The objective of this study was to compare Fe bioavailability from diets containing nonheme Fe (NHI), nonheme Fe with meat (...
Considering the significance of AGE-mediated protein modification by glyoxal, here we have investigated the in vitro effect of the reactive α-oxoaldehyde (10, 20μM) on the heme protein hemoglobin (HbA 0 ) (100μM) after incubation for one week at 25°C. In comparison with HbA 0 , ...
Overall, the use of biosolid as a sustainable soil amendment had positive effects not only on plant health and protection, but also on growth of non-pathogenic antagonistic microorganisms against Forl in the tomato rhizosphere and thus, on plant–soil microbiome interactions, toward biocontrol of ...
The serum triglyceride was measured by use of the enzyme method following the manufacturer’s instructions, and the absorption at the wavelength of 546 nm was detected to calculate the triglyceride (Triglyceride concentration = sample absorption value/calibrator absorption value × calibrator concentration...
of tuberous roots and malondialdehyde (MDA). The appropriate 5-ALA concentration in the late growth stage ofP. heterophyllaeffectively enhanced the net photosynthetic capacity, mainly resulting from the enhancement of PSII photochemical activity to promote the increases in excitation energy absorption, ...