增强型ATP检测试剂盒(Enhanced ATP Assay Kit)可以用于检测普通溶液、细胞或组织内的ATP水平。细胞和组织样品一步裂解即可完成。样品制备,检测灵敏度高达0.1nM,化学发光可以持续稳定达30分钟。本试剂盒获得的样品还可以同时进行Western检测。 ATP作为最重要的能量分子,在细胞的各种生理、病理过程中起着重要作用。ATP水平...
T1 cells presented loss of intracellular ATP, and 3BP@PLGA-IR780 led to a more significant ATP decline (Additional file1: Fig. S7). These metabolic interruptions subsequently led to strong cytocidal activities on tumor cells. Moreover, the mitochondrial-targeting ability of IR780 would be expect...
2.2.7. Intracellular ATP detection B16F10 cells were treated with PBS, Cu2+, Cu2++DSF, CLCu, and CLDCu (50 μg/mL, the other groups were dosed with the same concentrations of Cu2+ or DSF) for 24 h. An ATP assay kit was applied to evaluate intracellular ATP contents after cells ...
A cesium based internal solution containing (in mM) 135 CsMeSO4, 10 HEPES, 10 BAPTA, 5 Qx314, 0.3 NaGTP, 4 Na2ATP, 4 MgCl2, and 0.1 spermine, pH 7.25 with 1 M CsOH was used. Recordings were performed at room temperature and extracellular solution was exchanged at a flow rate of 3...
ATP content was determined by referring to the procedures of the ATP kit (Beyotime Institute of Biotechnology, China). Construction of monolayer-layer transwell model and evaluation of the performance of nanomotor penetrating BBB (1) 200 µL, bEnd.3 cells (cell density of 1.5 × 105...
An ATP assay kit (Beyotime S0026) was employed for the measurement of intracellular ATP level. Briefly, 4T1 cells (1 × 104) were seeded into 96-well plates and incubated at 37 °C for 24 h. The culture media were replaced with fresh ones containing 40 μM of TFR, TKFC and TRFC ...
Measurement of the ADP:ATP ratio The cellular energy status was assessed by measuring the ADP:ATP ratio, and ADP and ATP levels in the samples were determined using the ADP: ATP Ratio Assay Kit-Luminescence (Dojindo, Kumamoto, Japan). All samples were run as duplicates in three independent ...
4C). Metascape analysis revealed that the ATP metabolic process and response to oxygen levels pathway were enriched in NEAT1+ tumor cells (Fig. 4D and Additional file 1: Fig. S10A), where oxidative phosphorylation (OXPHOS)-related genes, COX1, COX2, COX3, ND1, ND2, ND4, and ATP6, ...
An ATP assay kit (Promega, Madison, WI) was used to measure the cellular ATP level according to the manufacturer’s instructions. Bioluminescence was determined on a fluorescence luminometer (PerkinElmer, Waltham, MA). The ATP level was calculated from a standard curve. A lactate assay kit (Bio...
Measurement of intracellular ATP levels Intracellular ATP levels in biopsy-derived kidney cells were measured using the CellTiter-Glo® 2.0 Assay kit and rATP standard (Promega, WI, USA). Six replicates of 10,000 live cells/well were seeded in white 96-well plates and incubated overnight at ...