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J Agric Food Chem 66(50):13217–13227 Article CAS Google Scholar Hughes AM, Darby JF, Dodson EJ, Wilson SJ, Turkenburg JP, Thomas GH, Wilkinson AJ (2022) Peptide transport in Bacillus subtilis–structure and specificity in the extracellular solute binding proteins OppA and DppE. Microbiology...
Non-structured regions extend before and after this motif, encompassing the signal sequence, Ccw12-characteristic repeats R1 and R2, and the C-terminal tail containing the ω-site (Fig. 1A). At the ω-site, GPI transamidase cleaves the polypeptide chain and covalently attaches the GPI anchor ...
Unfortunately, however, the ecological aspects of BGI – the fact that these are interconnected systems composed of living organisms – tend to be overlooked by the engineering community. This is regrettable, as there is evidence that biodiversity provides various ecosystem services (e.g., food prov...
These approaches can be used to harness microbiomes for broad applications related to medicine, agriculture, energy and the environment. We also discuss key challenges and opportunities of each approach and synthesize them into best practice guidelines for engineering microbiomes. We anticipate that ...
Microbial lipid metabolism is an attractive route for producing oleochemicals. The predominant strategy centers on heterologous thioesterases to synthesize desired chain-length fatty acids. To convert acids to oleochemicals (e.g., fatty alcohols, ketones
It is a single-chain protein. There are hydrophobic patches and voids, and it does not include any prosthetic groups; it is a simple protein, not glycosylated. There are three structurally similar domains that make up albumin. The structure has three domains: domain I, which spans residues 1...
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Over time, the cells exhibited global changes on both the transcriptomic and proteomic levels, including the shutdown of translation and cell motility, likely to conserve energy. These adaptations enabled the cells to maintain significant metabolic activity for several weeks. Acetate formation was shown...
Acetylation contributes to the bioactivity of numerous medicinally important natural products. However, little is known about the acetylation on sugar moieties. Here we report a saponin acetyltransferase from Astragalus membranaceus. AmAT7-3 is discovere