Engineering more stable proteinsElectronic supplementary information (ESI) available. See DOI: 10.1039/c8cs00014jNo AbstractKazlauskas, RomasCHEMICAL SOCIETY REVIEWS
Our generative model allows to learn a robust approximation of the sequence-to-free-energy function, which can then be exploited to generate more stable proteins. Specifically, PREVENT implements two free energy based design procedures, namely the seeded non optimal energy ranking (SNE) and the pri...
Directed evolution is a powerful method to optimize protein fitness. Here, authors develop an active learning workflow using machine learning to more efficiently explore the design space of proteins. Jason Yang ,Ravi G. Lal &Frances H. Arnold ...
Self-assembled monolayers (SAMs) are widely used to confine proteins and cells to a pattern to study cellular processes and behavior. To fully explore some... MK Strulson,DM Johnson,JA Maurer - 《Langmuir》 被引量: 36发表: 2012年 Increased Stability and Complexity of Glycol-Terminated Self-...
For instance, there are start-ups that are trying to create vaccines that are adapted for specific body proteins. There’s so much to build. There’s never been a more exciting time as a software developer, so keep building and learning. And the truth is that it’s never been easi...
1e). In addition, k0 and Δx‡ values measured using wild-type and mutated CohE proteins were similar (Table 1). We concluded that the introduction of azido-phenylalanine mutation and conjugation of Fgβ peptide did not affect the mechanical properties of the complex, and it is the change...
binders with high affinities, validated by binding assays. Moreover, we harness EvoPlay to prospectively engineer luciferase, resulting in the discovery of variants with 7.8-fold bioluminescence improvement beyond wild type. In sum, EvoPlay holds great promise for facilitating protein design to tackle...
Biomolecular condensates can serve as synthetic organelle-like compartments within prokaryotes. Here, the authors report a synthetic condensate platform, generated through the incorporation of artificial, disordered proteins, and demonstrate control of metabolic flux and protein translation in Bacillus subtilis...
The ancestral ketol-acid reductoisomerase showed an eight-fold higher specific activity than the cognate Escherichia coli form at 25 °C, which increased 3.5-fold at 50 °C. Thus, thermostable proteins can be devised using sequence data alone from even recent ancestors....
Accelerating anaerobic oxidation of methane coupled with extracellular electron transfer to electrodes via magnetite stimulating membrane-bound proteins of anaerobic methanotrophic (ANME) archaea/methanogens Lianfu Liang, Mingwei Wang, Qilin Yu, Zhiqiang Zhao, Yaobin Zhang Article 142266 Article preview select...