Endothelial cell culture: protocol to obtain and cultivate human um- bilical endothelial cells [J]. J Immunol Methods, 2001, 254(1-2): 183.Marin V,Kaplanski G,Gres S,et al.Endothelial cell culture: protocol to obtain and cultivate human umbilical endothelial cells. Journal of Immunological ...
Endothelial cell culture: protocol to obtain and cultivate human umbilical endothelial cells. 来自 NCBI 喜欢 0 阅读量: 143 作者:V Marin,G Kaplanski,S Grès,C Farnarier,P Bongrand 摘要: Endothelial cells play a key role in prominent immunological and pathological processes such as leukocyte ...
Objective:Objective: To investigate the effect of endothelial cells(ECs) on the differentiation of endothelial progenitor cells(EPCs) towards the mature ECs.Methods: Mononuclear cells(MNCs) were obtained from femur and tibia bone marrow of 1 to 2 month-old Sprague-Dawley rats by Ficoll density gra...
Sage, H. Collagen synthesis by endothelial cells in culture. In: E. A. Jaffe (ed.). Biology of Endothelial Cells, pp. 161-177. Boston: Martinus Nijhoff, 1984.Sage H. Collagen synthesis by endothelial cells in culture. In: Jaffe EA ed. The biology of endothelial cells. Amsterdam: ...
We have previously shown the presence of GpIbα and GpIbβ mRNA and protein in cultured human umbilical vein endothelial cells (HUVECs) as well as the presence of GpIbα mRNA and protein in tonsillar endothelium. We, therefore, probed ECs for the presence of the other components of the ...
Human lymphatic endothelial cellsIsolationForeskinLymphatic malformationMagnetic beadsCell cultureA protocol describing the isolation of foreskin lymphatic endothelial cells (LECs) and lymphatic malformation lymphatic endothelial cells (LM LECs) is presented herein. To isolate LECs and LM LECs, ti...
Molecular profile and proliferative responses of rat lymphatic endothelial cells in culture. BACKGROUND: Lymphangiogenesis plays an important role in metastasis of many solid tumors. To study lymphangiogenesis under controlled conditions, an in vit... B Whitehurst,C Eversgerd,M Flister,... - 《Lympha...
Studies using either animal models or in vitro flow systems have shown that the shape of large-vessel endothelial cells (ECs) was sensitive to the amplitude of the flow imposed on them. In order to better understand the morphological changes experienced by ECs when exposed to physical forces suc...
Protocol or Proli erating Cells 1. Incubate the cells Unpack the culture vessel, do not open the lid, and immediately place it in an incubator (37°C, 5% CO 2 ) or 3 hours to allow the cells to recover rom the transportation. 2. Replace the transport medium Care ully open the...
Isolation and culture of human endothelial cells.Endothelial cellsMicrovascularAdiposeDynabeadsPECAM-1/CD31von Willebrand FactorE-selectinThe endothelium from different vascular beds exhibits a high degree of phenotypic heterogeneity. Endothelial cells (EC) can be harvested easily from large vessels by ...