cell interactions in vitroadhesion of leukocyteshigh endothelial venulesHEV assaycultured endotheliumadhesion assay under static conditionsThesis (M.Lit.St.)--University of Queensland. Includes bibliography.doi:10.1002/352760779X.ch17Markus HammelOlaf Zilles...
产品名称: QCM Endothelial Cell Invasion Assay (24-well, colorimetric) .0 订购此产品 供应商: Millipore 规格: EA 目录价: 5487 库存状态: 三周到货 CAS编号: 应用范围: 生化实验 种属来源: 相关信息: QCM™ Endothelial Cell Invasion Assay (24 well, colorimetric) Close PRODUCT FAMILY INFORMATION...
Monocyte–endothelial cell adhesion assay HUVECs were pretreated with DA (10 nM), anti-ICAM-1 Ab (10 μg/mL), or other inhibitors for 1 h followed by TNF-α (10 ng/mL) treatment for 24 h. THP-1 cells labeled with Hoechst 33342 were co-incubated with HUVECs for 3 h...
of the cells in the blood, for example,monocyteson thesurfaceof the endothelial cells. Such molecules includevascular cell adhesion molecule(VCAM) and platelet endothelial cell adhesion molecule (PECAM), although the role of this latter molecule in atherosclerosis is complex (Getz and Reardon, 2016...
ARM-1 mRNA was found to be differentially expressed in endothelial cell lines of various tissue origin and lymphocyte cell lines. Interestingly, ARM-1 is absent from skin endothelial cells. In our assay, skin endothelial cells display a distinct capacity to mediate adhesion of activated T...
2.3 Cell viability assay Cell viability was determined by using the CellQuanti-Blue™ assay (BioAssaySystems, Hayward, CA, USA) according to the manufacturer’s instructions. Briefly, cell viability was assessed via measuring the metabolic activity by the reduction of the substrate resazurin to res...
necrophorum mediate adhesion to bovine cells. Section snippets Bacterial strains and culturing for adhesion assay F. necrophorum subsp. necrophorum, strain A25 and F. necrophorum subsp. funduliforme, strain B35, previously isolated from liver abscesses of cattle (Lechtenberg et al., 1988), were...
Since ECs were activated by pro-inflammatory cytokines we complemented the previous results with a monocyte-EC adhesion assay to determine whether adhesion molecules were functional in mediating monocyte adhesion to ECs and to determine whether this activity was different between arterial and venous ECs31...
Cell pellets were lysed in appropriate volumes of lysis buffer to generate extracts containing equal number of cells per unit volume. A fluorometric based assay for caspase-3 activity was performed using Ac-DEVD-AMC as a substrate as described in the methods section. Bars are representative of ...
A thieno[2,3-d]pyrimidine, A-155918, was identified from a whole-cell high-throughput assay for compounds which inhibited the tumor necrosis factor-alpha (TNFalpha)-induced expression of E-selectin, ICAM-1, or VCAM-1 on human vascular endothelial cells. Traditional medicinal chemistry methods ...