PURPOSE: To obtain a new enzyme useful for cleaving and removing an O- glycoside binding saccharide chain in a glycoprotein having the saccharide chain containing sialic acid.TOCHIKURA TATSUROKURO栃倉 辰六郎KUMAGAI HIDEHIKO熊谷 英彦YAMAMOTO KENJI山本 憲二KOJIMA ICHIRO小島 一郎...
;CONSTITUTION: endo-α-N-Acetylgalactosaminidase. substrate specificity: capable of acting on a glycoprotein, composed of two or more saccharides having a basic structure of Galβ1 → 3GalNAcα 1 → Ser(Thr) [Ser(Thr) is serine residue or threonine residue in protein] and capable of acting...
PURPOSE:To efficiently produce endo-alpha-N-acetylgalactosaminidase, by cultivating a microorganism, belonging to the genus Alcaligenes and having the ability to produce the endo-alpha-N-acetylgalactosaminidase. CONSTITUTION:A microorganism, e.g. F-1906 strain separated from soil, belonging to the ...
This invention provides endo- alpha -N-acetylgalactosaminidase (endo- alpha -GalNAcase) from a microorganism belonging to the genus Alcarigenes. This endo- alpha -GalNAcase is very useful in the analysis of the structure and function of mucin-type sugar chains of glycoproteins, as it is an...
Tatsurokuro TochikuraDepartment of Food Science and TechnologyAgricultural and Biological ChemistryYamamoto K, Fan J-Q, Kadowaki S, Kumagai H, Tochikura T, Isolation of endo-α- N -acetylgalactosaminidase from an aerobic bacterium, Agric Biol Chem 51(11):3169- 3171, 1987....
PROBLEM TO BE SOLVED: To obtain the subject enzyme useful for the investigation of the structure and function of a glycoprotein glucide by culturing a microorganism belonging to the genus Aureobacterium and collecting endo-α-N-acetylgalactosaminidase from the cultured product.MOCHIZUKI KAZUO...
Ito. 2008. Molecular cloning, expression, and characterization of a novel endo-alpha- N-acetylgalactosaminidase from Enterococcus faecalis. Biochem. Biophys. Res. Commun. 375:441-446.Goda HM, Ushigusa K, Ito H, Okino N, Narimatsu H, Ito M (2008) Molecular cloning, expression, and ...
“Efficient synthesis of O-linked glycopeptide by a transglycosylation using endo &agr; -N-acetylgalactosaminidase from streptomycessp.”, Biosci. Biotechnol. Biochem., (2001) vol. 65(5): 1240-1243. ;., (2001) vol. 65(5): 1240-1243....
BARDALES R.M. ET AL.: ' Transglycosylation and transfer reaction activities of endo-alpha-N-acetyl-D-galactosaminidase from Diplococcus (Streptococcus) pneumoniae ' J. BIOL. CHEM. vol. 264, no. 33, 1989, pages 19893 - 19897, XP002216058...
PROBLEM TO BE SOLVED: To obtain the subject enzyme useful for the investigation of the structure and function of a glycoprotein glucide by culturing a microorganism belonging to the genus Aureobacterium and collecting endo-α-N-acetylgalactosaminidase from the cultured product. ;SOLUTION: The endo-...