levels are increased by a post-transcriptional mechanism that involves the binding of PT cytosolic proteins to the PTH mRNA 3’-untranslated region (UTR)... J Silver,R Kilav,SB Naveh-Many - 《Pediatric Nephrology》 被引量: 97发表: 2000年 Multiple transcription start sites and alternative splic...
Premature transcription termination yields a wealth of unadenylated (pA−) RNA. Although this can be targeted for degradation by the Nuclear EXosome Targeting (NEXT) complex, possible backup pathways remain poorly understood. Here, we find increased levels of 3′ end uridylated and adenylated RN...
termed the Ac-N-end rule, is responsible for the recognition and degradation of proteins that contain an acetylated N-terminal residue (Fig. 1B). Although it was known for more than 20years that some N-terminally acetylated proteins could be degraded by the ubiquitin system, it was ...
(Table S6), the most significantly enriched GO terms were protein binding (GO: 0005515) with 154 genes annotated, followed by ATP binding (GO: 0005524), cal- cium ion binding (GO: 0005509), metal ion binding (GO: 0046872), and sequence-specific DNA binding transcription factor activity (...
During the repair of interstrand crosslinks (ICLs) a DNA double-strand break (DSB) is generated. The Fanconi anemia (FA) core complex, which is recruited to ICLs, promotes high-fidelity repair of this DSB by homologous recombination (HR). However, whethe
It should be noted that Zm00001d047250 (B-PEG in endosperm of BC/CB) and Zm00001d024810 (B-MEG in endosperm of BC/CB) were PLATZ transcription factor 14 (platz14) and MYB- related transcription factor 33 (mybr33). The GO enrichment of ASIGs To explore the function of ASIGs in ...
Zero Blunt TOPO PCR Cloning provides a highly efficient, 5 minute, one-step cloning strategy ("TOPO Cloning") for the direct insertion of blunt-end PCR products into a plasmid vector. No ligase, post-PCR procedures, or PCR primers containing specific ...
transcription complex (RTC) to synthesize genomic RNA (gRNA) and sgRNAs. Coronavirus PRF is regulated by a frameshift element upstream of the ORF1a stop codon35. We used PRF as a surrogate assay to determine the influence of the SPEAR on RTC formation by comparing PRF efficiency of a SPEAR...
1b). Then the frequency of each of these two repair mechanisms was calculated (see “Methods”). Fig. 1: Assay to study RNA-mediated DSB repair in human cells. a Schemes of sense and antisense-genetic constructs expressing RNA transcripts that differ by sequence and transcription level. (...
Class-switch recombination: interplay of transcription, DNA deamination and DNA repair. Nat. Rev. Immunol. 4, 541–552 (2004). CAS PubMed Google Scholar Mimitou, E. P. & Symington, L. S. Ku prevents Exo1 and Sgs1-dependent resection of DNA ends in the absence of a functional MRX ...