Enantiomeric purityRoscovitineA precise and sensitive LC method for the determination of roscovitine enantiomeric purity has been developed and validated. Baseline separation with a resolution higher than 5.8 was accomplished within 15 min using a CHIRALPAK AD column (250 × 4.6 mm; particle size 5 ...
Krecz, I.Almás, M.Somlai, Cs.Taylor & Francis GroupJournal of Liquid ChromatographySzoka´n Gy, Hadafi Sz, Krizsan K, Liembeck A, Krecz I, Almas M, Somali Cs (1994) HPLC determination of enantiomeric purity of protected amino acid derivatives used in peptide synthesis. J Liq ...
Enantiomeric purityPF-04136309A precise and sensitive LC method was developed and further validated for the determination of enantiomeric purity of ( S )- N -[2-(3-{ trans -4-hydroxy-4-[5-(pyrimidin-2-yl)pyridin-2-yl] cyclohexylamino} pyrrolidin-1-yl)-2-oxoethyl]-3-(trifluoromethyl) ...
A sensitive and specific analytical method was developed to determine the enantiomeric purity of naproxen. A simple derivatization of naproxen with (S)-(+)-2-octanol proceeded quantitatively and gave a mixture of diastereomeric esters displaying baseline separation on liquid chromatography. The ratio of...
Enantiomeric purity determinationChiral liquid chromatographyA simple and reliable isocratic normal phase HPLC method for the determination of thymoquinone in black seed oil (Nigella Sativa Linn, Ranculaceae) is described. After oil extraction with methanol, thymoquinone is analyzed using Econosphere CN ...
Optica purity detected by po arimeter HPLC 法测定的光度纯度(%) Enantiomeric excess detected by HPLC 相对偏差 ”” (%) Re ative deviation 1 9. 86 9. 74 1. 22 2 29. 8 29. 4 1. 34 3 50. 0 49. 5 1. 10 4 70. 1 69. 4 1. 03 5 90. 4 89. 6 0. 85 ”:光学纯度按标准...
have been developed and successfully applied for the enantiomeric purity determination. Among them, NMR is a still useful method, although most of the enantiomer separation of chiral drugs has been performed by HPLC or CE. In the NMR method, chiral derivatizing reagents (CDRs) or chiral shift ...
method and those determined by chiraI HPLC were Iess than 3. 0%. The proposed method has a high accuracy and shows a good perspective to determine the ee of chiraI compounds. Keywords CircuIar dichroism,high performance Ii uid chromatography,enantiomericpurity,anisotropy factor,metaIaxyI (Received...
With a mobile phase of IPA/hexane/HAc/triethylamine (15/85/0.2/0.05, V/V, flow rate 0.4 mL/min), the resolution of ibuprofen enantiomers was sufficient for precise enantiomeric purity determination.Xie YuchunLiu HuizhouChen Jiayong谢渝春
Analytical enantioseparation methods using UV detection were validated to determine the enantiomeric purity of these compounds. Linear calibration curves in the range from 0.18 to 0.40 mM were obtained; repeatability, limits of detection (LOD), and quantification (LOQ) were determined: LOD varied, for...