2.5. Measurement of Citrate Synthase Activity Total proteins were purified from 15 mg of frozen heart samples by homogenization in a buffer containing 100 mM mannitol, 1 mM ATP, 0.2% bovine serum albumin (BSA), 100 mM KCl, 3 mM MgCl2, 5 mM Tris-buffer, 1 mM EDTA, pH 7.4. Protein ...