HER2 在正常情况下表达低或不表达,但在各种实体 瘤中经常过表达,HER2 与多种癌症相关,包括乳腺癌、胃癌、结肠癌、膀胱癌、卵巢癌、 子宫内膜癌、肺癌等。 II.Introduction Host Cell:EMT6 Expressed gene:hHER2 Stability:32 passages (in-house test, that not means the cell line will be instable beyond ...
Fig2.In vivo validation of EMT6-hHER2-Luc in BALB/c mice Fig3.HER2 expression in tumors after in vivo growth of EMT6-hHER2-Luc in wild type BALB/c mice 相关品系 Rb1-Flox/Trp53-Flox/R26-CAG-LSL-Luc-2A-EGFP 细胞系名称:Rb1-Flox/Trp53-Flox/R26-CAG-LSL-Luc-2A-EGFP ...
Fig2. In vivo validation of EMT6-hHER2-Luc in BALB/c mice Fig3. HER2 expression in tumors after in vivo growth of EMT6-hHER2-Luc in wild type BALB/c mice 相关品系 Rb1-Flox/Trp53-Flox/R26-CAG-LSL-Luc-2A-EGFP 细胞系名称:Rb1-Flox/Trp53-Flox/R26-CAG-LSL-Luc-2A-EGFP ...
摘要: 目的:构建人表皮生长因子受体(HER2)胞外区(1896bp)基因的真核表达质粒(pcDNA6/v5 his HER2),转染小鼠乳腺癌细胞(EMT6),获得其稳定表达细胞株(EMT6/HER2)。方法:用PCR方法从含HER2全长基因的pcDNA3.1 HER2质粒上扩增HER2胞外区基因序列;经酶切、连接构建pcDNA6/v5 his HER2;转化大肠杆菌DH5α,筛选阳性...
英文名称 EMT6-ffluc-GFP+HER2(h) 稳转株 产品货号 VGC-0666-021P 中文名称 HER2(h) 过表达 EMT6 稳转株 Lot number EMT6-24D4-32B6P 细胞规格 复苏细胞 (T25细胞瓶) 或1 × 106 细胞冻存细胞 细胞形态 上皮细胞样 生长特性 贴壁生长 细胞株 构建流程 (1)构建目的基因过表达慢病毒重组质粒,并包装...
以PEI法将pcDNA6/v5-his- HER2导入EMT6小鼠乳腺癌细胞,经杀稻瘟菌素(Blasticidin)筛选1～2周,获得抗性克隆EMT6/HER2;用RT-PCR检测 EMT6/HER2中HER2 mRNA,免疫组化法检测其HER2蛋白的表达.结果:PCR产物与预期片段大小一致;pcDNA6/v5-his-HER2经酶切,琼脂糖凝胶电泳后, 可见与PCR产物大小相同的片段;DNA测序结果...
资料)文1:重组HER2真核表达载体的构建及其稳定转染EMT6细胞株的筛选临床医学[Abstract]AIM: To cotruct an eukaryotic vector encoding extracellular domain of human epidermal growth factor recepto (HER2), pcDNA6/v5 his HER2, and to screen HER2 positive clones from mouse breast cancer cell line EMT6. ...
方法: 用PCR方法从含HER2全长基因的pcDNA3.1 HER2质粒上扩增HER2胞外区基因序列; 经酶切、 连接构建pcDNA6/v5 his HER2; 转化大肠杆菌DH5α, 筛选阳性克隆, 对其进行酶切及测序鉴定; 以PEI法将pcDNA6/v5 his HER2导入EMT6小鼠乳腺癌细胞, 经杀稻瘟菌素(Blasticidin)筛选1～2周, 获得抗性克隆EMT6/HER2; 用...
896bp)基因的真核表达质粒(pcDNA6/v5hisHER2),转染小 鼠乳腺癌细胞(EMT6),获得其稳定表达细胞株(EMT6/HER2)。方 法:用PCR方法从含HER2全长基因的pcDNA3.1HER2质粒上 扩增HER2胞外区基因序列;经酶切、连接构建 pcDNA6/v5hisHER2;转化大肠杆菌DH5α,筛选阳性克隆,对 ...
Fig2. In vivo validation of EMT6-hHER2-Luc in BALB/c mice Fig3. HER2 expression in tumors after in vivo growth of EMT6-hHER2-Luc in wild type BALB/c mice 相关品系 Rb1-Flox/Trp53-Flox/R26-CAG-LSL-Luc-2A-EGFP 细胞系名称:Rb1-Flox/Trp53-Flox/R26-CAG-LSL-Luc-2A-EGFP ...