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respect and dignity are imperative for both patients and staff. Management worked hard as well and were fair. They were willing to go above and beyond if you needed extra training. Culture was diverse for both patients and staff. The hardest partof the job was not knowing what time you wer...
The mRNA was analyzed by qRT-PCR in whole hearts from P0 mice. ∗p < 0.05 versus Tbx18-Cre;Prmt1fl/+;ROSAYFP/+. (D–F) cVSMC formation was reduced by epicardial-specific Prmt1 deletion. cVSMCs were revealed by αSMA immunostaining in P0 hearts and ECs were revealed by CD31, as ...
KrasG12D;p53fl/fl lung adenocarcinoma cells were infected with retroviral vectors expressing rtTA3, luciferase, and a tet-on shKras. Cells were transplanted into recipient mice by tail-vein injection. At 7 days later, mice were fed a doxycycline diet to induce shKras in tumor cells (D0)....
Clumps were removed by additional rounds of straining until a single cell suspension was obtained. Single cells were seeded at a concentration of B18 cells/mm2 (B105 cells in a 100-mm dish, 55 cm2 surface area) in ULA dishes (no. 3262, Corning Inc.). Cell suspension was incubated over...