Linked ligation was carried out as follows: 23 μL of the above DNA, 3 μL 10X T4 DNA ligase buffer, 1 μL Illumina Index adapter oligo mix, and 3 μL T4 DNA ligase. The adapter reaction was incubated for 30 min at room temperature. Size selection was carried out using ...
Flexible conduit is ribbed, giving it a look similar to corrugated wire loom, and is able to bend and flex freely (though it does not hold a bend permanently as a bent pipe would). For areas where obstacles would require rigid conduit to make too many turns and use an impractical number...
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