EMSA analysis of Rgg protein activity has facilitated in vitro confirmation of regulatory targets, identification of precise DNA binding sites via DNA probe mutagenesis, and characterization of the mechanism by which some cognate signaling peptides modulate Rgg protein function (e.g. interruption of ...
撰写文章时，该实验可引用以下参考文献Li W, Zhu Z, Chern M, et al. A Natural Allele of a Transcription Factor in Rice Confers Broad-Spectrum Blast Resistance. Cell. 2017;170(1):114-126.e15.LaSarre B, Federle MJ. EMSA Analysis of DNA Binding By Rgg Proteins. Bio Protoc. 2013;3(15...
撰写文章时,该实验可引用以下参考文献 Li W, Zhu Z, Chern M, et al. A Natural Allele of a Transcription Factor in Rice Confers Broad-Spectrum Blast Resistance.Cell. 2017;170(1):114-126.e15. LaSarre B, Federle MJ. EMSA Analysis of DNA Binding By Rgg Proteins.Bio Protoc. 2013;3(15)...
7、troductionmany regulatory steps in cellular processes, such as replication and transcription, depend upon the binding of proteins to specific dna sequences. such protein-nucleic acid interactions are commonly studied by filter binding, dna footprinting, methylation interference, and gel shift assays ...