The mutation frequency in the library (~1 substitution per 100 kbp) is comparable to those reported for other high-mutation-frequency libraries. Thus, the present library has a potential to yield useful mutant alleles for soybean breeding.Mai Tsuda...
关键词 甲基磺酸乙酶;突变体库;种质资源;叶夹角 中图分类号 5188 文献标识码 A 文章编号 0517 -6611 (2014)11 -03162-04 Construction and PreIiminary Identification of EMS Mutant Library in M凶ze FAN Shuang-hu , ZBANG Chun-yi et al ( School of Life Science and Engineeri吨, Southwest ...
Atthesametimeꎬtheresearchprogressof EMSmutagenesistechnologyindiseaseresistancegenecloningꎬqualitytraitimprovementꎬleafgreeningmechanismre~ searchꎬagronomictraitanalysisꎬandmutantlibraryconstructionwasreported. Theproblemsandcountermeasuresof EMSmutagenesisinwheatresearchwereanalyzed. ThedevelopmentprospectofEMS...
primers spanning exons II to IX were used to isolate transcripts from theSBEIIa-B-2mutant. PCR amplification resulted in two bands of different size: the larger product showed the inclusion of the intron V, whereas the smaller one was found to contain a...
图1 通过EMS诱变构建二穗短柄草突变体库Fig.1 The construction for mutant library of brachypodium distachyon by EMS mutagenesis 3 EMS诱变在植物育种中的应用实例 EMS诱变技术已作为一种有效的手段广泛应用于植物品质改良与品种选育上,并且创制出了大批具有高产、优质、早熟、矮秆、抗病等农艺性状的新材料和新品种。
摘㊀要:小麦遗传基础日趋狭窄成为小麦遗传改良的瓶颈ꎮ甲基磺酸乙酯(ＥＭＳ)是一种高效稳定的烷化类诱变剂ꎬ能诱发产生高密度系列等位基因点突变ꎬ可在创造作物新品种㊁新种质㊁遗传材料以及解决育种工作中某些特殊问题等方面取得突破ꎮ本文从ＥＭＳ诱变的原理和特点着手ꎬ简述了小麦ＥＭＳ诱变及突变体鉴...
mutant librarymorphological characterizationyellow leafWatermelon is a crucial horticultural crop worldwide but its genetic base has become extremely narrow owing to long-term cultivation. Induced mutagenesis can create a range of variations with distinctive agricultural characteristics. To broaden the genetic...
Optimization of EMS mutagenesis condition and construction of mutant library in flowering Chinese cabbage ZHANG Yan, HONG Xiao-ru, LI Ting-yao, SHEN Zhuo, YANG Yi, ZHOU Xuan, CHEN Han-cai* (Vegetable Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for New Te...