EMS mutagenesis of Arabidopsis. Methods in Molecular Biology, New York, v. 323, p. 101-103. 2006.Kim Y, Schumaker KS, Zhu JK. (2006) EMS mutagenesis of Arabidopsis. Meth Mol Biol. 323:101‐103....
emsmutagenesisthiosulphate种子hpo诱变 EMS诱变一溶液配制11L0.1Mthiosulphatesolution取24.818gsodiumthiosulphate(sigma,Na2S2O3·5H2O分子量248.18)溶液到ddH2O中,至终体积1L。20.1MKPO3buffer(pH7.5)先配1MK2HPO4和1MKH2PO4母液,1MK2HPO4:MW174.17,8.7085gK2HPO4溶解到ddH2O中,终体积50mL1MKH2PO4:MW136.09,6.804...
EMS Mutagenesis of Arabidopsis
利用EMS进行茄子种质资源创新%Innovation of new eggplant germplasm by EMS mutagenesis利用EMS进行茄子种质资源创新%Innovation of new eggplant germplasm by EMS mutagenesis茄子EMS诱变种质资源性状突变创新[目的]利用EMS诱变进行茄子种质资源创新,为茄子新品种选育及其相关基因功能研究提供良好材料.[方法]在25℃下利用...
Mol Plant.2025 Mar 4:S1674-2052(25)00091-7.doi:10.1016/j.molp.2025.03.001.[5] Settles A M. EMS Mutagenesis of MaizePollen[M]. 2020.[6]杨媛媛.玉米EMS突变体库创建及opaque和CENH3基因的TILLING分析[D].四川农业大学,2013. 送TA礼物 1楼2025-04-25 11:28回复 ...
Keywords Wheat;EMS mutagenesis;Mutant;Agronomic traits;Disease resistance 小麦是世界三大粮食作物之一,世界上超过三分之一的人口以小麦为主粮。在我国,小麦是北方人口的主要食粮,每年种植面积约2 300万公顷,占全国粮食消费总额的1/5以上,居第三位,仅次于水稻和玉米[1]。近年来,随着我国人口快速增长,城市化进程加快...
EMS Mutagenesis,Mutant Screening and Identification of Sorghum Wang Chunyu,Zhu Zhenxing, Li Dan, Cong ling,Zhang Lixia 生物技术通报 . 2014, (9): 78 -83 . 网站版权 © 《生物技术通报》编辑部 地址:北京市中关村南大街12号 邮编:100081 电话:86-010-82109925, 82109903 Email:biotech@caas.cn 本...
plant rate of two materials were 13% and 31% respectively. When EMS concentration was 0.8%, the adult plant rate of C40 decreased to 50%, and the seed-setting plant rate of both materials was less than 20%. Under the same concentration of EMS, the longer the mutagenesis time was, the...
Key words:Broccoli; Seed; EMS mutagenesis; Germination rate; Phenotype 西蓝花(Brassica oleracea var. italica)又称绿花菜、青花菜等,属十字花科甘蓝的一个变种,其口感佳,且富含萝卜硫苷、蛋白质、维生素C等物质,因而有“蔬菜皇冠”之称[1-3]。西藍花起源于地中海,近年来在中国的栽培面积快速增长。目前,西...
TILLING (Targeting Induced Local Lesions IN Genomes) is a reverse genetic method that combines chemical mutagenesis with high-throughput genome-wide screening for point mutation detection in genes of interest. However, this mutation discovery approach fa